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Plasmid#105790PurposeExpression of your protein of interest in fusion with cyan fluorescent protein at the C-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 14990965, 17040988, 22264545).DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-mCeruleanExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only
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pKK-mCerulean-TEV
Plasmid#105773PurposeExpression of your protein of interest in fusion with cyan fluorescent protein at the N-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 14990965, 17040988, 22264545).DepositorTypeEmpty backboneUseFlp-in competentTagsmCerulean-TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCerulean-mVenus-FRET-10
Plasmid#58151PurposeLocalization: Cytosolic FRET, Excitation: 433 / 515, Emission: 475 / 528DepositorTypeEmpty backboneUseTagsmCerulean and mVenusExpressionMammalianMutationPromoterAvailable sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
paavCAG-pre-mGRASP-mCerulean
Plasmid#34910PurposepreDepositorInsertpresynaptic mGRASP
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceNov. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
765 pME nls mCerulean
Plasmid#195958PurposeGateway pME fluorophoreDepositorInsertmCerulean nuclear
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Hygro DEST ERKKTRmCerulean3
Plasmid#90229PurposeLentiviral vector to express ERK KTR mCerulean3 under PGK promoter (With Hygromycin Resistance)DepositorInsertERK Kinase Translocation Reporter
UseLentiviralTagsmCerulean3ExpressionMammalianMutationPromoterPGKAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Puro DEST JNKKTRmCerulean3
Plasmid#90232PurposeLentiviral vector to express JNK KTR mCerulean3 under PGK promoter (With Blasticidin Resistance)DepositorInsertJNK Kinase Translocation Reporter
UseLentiviralTagsmCerulean3ExpressionMammalianMutationPromoterPGKAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLentiCMV Puro DEST p38KTRmCerulean3
Plasmid#59155PurposeLentiviral vector to express p38 KTR mCerulean3 under CMV promoter (With Puromycin Resistance)DepositorInsertp38 Kinase Translocation Reporter (MAPK14 Mouse, Human)
UseLentiviralTagsmCerulean3ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
raACR-mCerulean3-pAAV2-hSyn
Plasmid#204966PurposePanneuronal expression of the MsACR1 mutant raACR fused to mCerulean3DepositorInsertraACR
UseAAVTagsmCerulean3ExpressionMutationPromoterhSynAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRRLNeo-pEF1a-p53ashL344A-mCerulean
Plasmid#69580PurposeExpresses p53-L344A mutant tagged with mCeruleanDepositorInsertp53-L344A (TP53 Human)
UseLentiviralTagsmCeruleanExpressionMammalianMutationp53 L344A mutation that prevents tetramerization …PromoterEF1alphaAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
hSyn-DIO-somBiPOLES-mCerulean
Plasmid#154951PurposeOptogenetic tool for blue-light inhibition and red-light excitation of neuronsDepositorHas ServiceAAV Retrograde, AAV5, and AAV9InsertsomBiPOLES
UseAAVTagssoma-targeting motif from Kv2.1 channelExpressionMammalianMutationPromoterhuman synapsinAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAF018 p3E-2A-mCerulean-CAAX
Plasmid#195975PurposeGateway p3E 2A fluorophoreDepositorInsert2A-mCerulean-CAAX (membrane)
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
hSyn-DIO-BiPOLES-mCerulean
Plasmid#154950PurposeOptogenetic tool for blue-light inhibition and red-light excitation of neuronsDepositorInsertBiPOLES
UseAAVTagsExpressionMammalianMutationPromoterhuman synapsinAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
769 pME mCerulean-CAAX
Plasmid#195957PurposeGateway pME fluorophoreDepositorInsertmCerulean-CAAX (membrane)
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ef1a-DIO-BiPOLES-mCerulean
Plasmid#154949PurposeOptogenetic tool for blue-light inhibition and red-light excitation of neuronsDepositorInsertBiPOLES
UseAAVTagsExpressionMammalianMutationPromoterEf1-alphaAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
His6-TEV-mCerulean3-pBluescript
Plasmid#166757PurposeFor purification of mCerulean3 fluorophoreDepositorInsertmCerulean3
UseTags6x Histidine tag, and TEV protease siteExpressionBacterialMutationPromoterT7Available sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Luciferase-IRES-mCerulean
Plasmid#75022PurposeEncodes the expression of Luciferase and mCeruleanDepositorInsertLuciferase
UseLuciferase and RetroviralTagsExpressionMutationPromoterAvailable sinceJune 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRRLNeo-pEF1a-p53ashL344P-mCerulean
Plasmid#69581PurposeExpresses p53-L344P mutant tagged with mCeruleanDepositorInsertp53-L344P (TP53 Human)
UseLentiviralTagsmCeruleanExpressionMammalianMutationp53 L344P mutation that prevents oliogomerization…PromoterEF1alphaAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Beta-2-adrenergic receptor-mCerulean
Plasmid#66957PurposeBeta-2-adrenergic receptor tagged with mCerulean on carboxyl terminusDepositorInsertBeta-2-adrenergic receptor-mCerulean (ADRB2 Aequorea victoria, Human)
UseTagsmCeruleanExpressionMammalianMutationPromoterCMVAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC19.TRIM28.FKBP.knock-in.mCerulean
Plasmid#185009PurposeFor knocking in FKBP F36V P2A mCeruleanDepositorInsertFKBP F36V P2A mCerulean
UseCloningTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only