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Plasmid#218273PurposeCRISPR KO plasmid for ALDH7A1 in human cell linesDepositorInsertInserted sgRNA targetting ALDH7A1 (ALDH7A1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRRv2_sgTom
Plasmid#218346PurposeCRISPR KO plasmid for tdTomato in human cell linesDepositorInsertInserted sgRNA targetting tdTomato
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV_Puro_SFB_GNB1L
Plasmid#224381PurposeLenti plasmid for generating GNB1L expressing stable cell linesDepositorInsertGNB1L (GNB1L Human)
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRDA52_Lenti.puro.U6_modified DR
Plasmid#196723PurposeFor cloning pairs of AsCas12a guides with two DRs that are as different as possible.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLentiGuide_mCherry_NT135
Plasmid#183121PurposepLentiGuide modified to express mCherry and a non-targeting sgRNADepositorInsertNT135
UseLentiviralTagsExpressionMutationPromoterU6Available sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv1_COX4
Plasmid#177983Purposelentiviral vector expressing Cas9 and a sgRNA targeting COX4DepositorInsertsgRNA targeting COX4
UseLentiviralTagsExpressionMammalianMutationN/APromoterU6Available sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti_CRISPRi_sgAR10
Plasmid#167003PurposeLentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdownDepositorInsertAR Promoter sgRNA 10 (AR Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_RPS10_K139R
Plasmid#127137DepositorInsertRPS10 (RPS10 Human)
UseLentiviralTagsExpressionMutationK139RPromoterAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_RPS10_K138R
Plasmid#127136DepositorInsertRPS10 (RPS10 Human)
UseLentiviralTagsExpressionMutationK138RPromoterAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_RPS2_K58R_Keima
Plasmid#127141DepositorInsertRPS2_Keima (RPS2 Human)
UseLentiviralTagsExpressionMutationK58RPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_RPS2_K275R
Plasmid#127131DepositorInsertRPS2 (RPS2 Human)
UseLentiviralTagsExpressionMutationK275RPromoterAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_RPS3_K227R_Keima
Plasmid#127144DepositorInsertRPS3_Keima (RPS3 Human)
UseLentiviralTagsExpressionMutationK227RPromoterAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_PROX1_1+4
Plasmid#121177PurposeLentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.DepositorInsertCas9-P2A-puro and U6 promoters driven expression of gRNAs
UseCRISPR and LentiviralTagsP2A-puroExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti_Neo_WT_ZFP64_CR
Plasmid#118694PurposeExpress sgZFP64e2 resistant cDNADepositorInsertZFP64 (ZFP64 Human)
UseLentiviralTagsExpressionMutationsgZFP64e2 targeting site was synonymous mutatedPromoterEF1aAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_ZFP64_Delta_TAD
Plasmid#118698PurposeExpress ZFP64 without transcription activation domain(TAD)DepositorInsertZFP64_Delta_TAD (ZFP64 Human)
UseLentiviralTags3XFLAG was fused to the N terminal of ZFP64 cDNAExpressionMutationDelete amino acid from 453 to 681PromoterAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti_Neo_ZFP64_N_3XFLAG
Plasmid#118700PurposeExpress 3xflag tagged ZFP64DepositorInsertZFP64 (ZFP64 Human)
UseLentiviralTags3XFLAG was fused to the N terminal of ZFP64 cDNAExpressionMutationPromoterEF1AAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
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LentiV_Neo_LKB1_CR
Plasmid#108112PurposeLentiviral expression plasmid of human LKB1 cDNA (CRISPR-resistant silent mutation) with neomycin resistance geneDepositorInsertLKB1 (STK11 Human)
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationchange cytosine 333 to alanine (silent mutation)PromoterEFS promoterAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2 blast
Plasmid#98293PurposeVariant of lentiCRISPRv2 that confers blasticidin S resistanceDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterEF-1aAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only