Addgene

We narrowed to 729 results for: ha/1000

Showing: 181 - 200 of 729 results

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pSCALPS-FLAG-HA-mmuMettl3 APPA

Plasmid

#207124

Purpose

Lentiviral plasmid to express mouse Mettl3 APPA mutant

Depositor

Silvia Monticelli

Article

Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

Insert

Mettl3 (Mettl3 Mouse)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mutation

D395A and W398A

Promoter

SSFV

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pSCALPS-FLAG-HA-mmuMettl3-LN

Plasmid

#207129

Purpose

Lentiviral plasmid to express mouse Mettl3 Lambda peptide C-ter

Depositor

Silvia Monticelli

Article

Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

Insert

Mettl3 (Mettl3 Mouse)

Use

Lentiviral

Tags

FLAG-HA and Lambda Peptide (LN)

Expression

Mutation

Promoter

SSFV

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA eIF4GI (1-1599)

Plasmid

#45640

Depositor

Nahum Sonenberg

Article

Yanagiya et al Mol Cell Biol. 2009 Mar;29(6):1661-9. do

Insert

eIF4GI transcript variant 2 (EIF4G1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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HA-HIF1alpha P402A/P564A-pcDNA3

Plasmid

#18955

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 1 alpha (HIF1A Human)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 402 to Alanin…

Promoter

Available since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-HA-MEK5 CBM-E

Plasmid

#53168

Purpose

Expresses GST-HA tagged human MEK5 L280AH281AM323AQ332A from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK5 (MAP2K5 Human)

Use

Tags

GST and HA

Expression

Bacterial

Mutation

L280A/H281A/M323A/Q332A

Promoter

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
pLenti-HA-CBS(opt)-FLAG

Plasmid

#230088

Purpose

expressing human CBS

Depositor

Xingguo Zhu

Article

Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

Insert

CBS (CBS Human)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Multiple wobble mutations

Promoter

CMV

Available since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pWZLblasti-HA-MEK5 CBM-E

Plasmid

#53169

Purpose

Expresses HA tagged human MEK5 L280AH281AM323AQ332A from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK5 (MAP2K5 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

L280A/H281A/M323A/Q332A

Promoter

Available since

Sept. 19, 2014

Availability

Academic Institutions and Nonprofits only
pENAAV-cTNT-actn2-BioID2-HA

Plasmid

#216831

Purpose

cardiomyocytes-specific plasmid, expressing the coding sequence of mouse Actn2, biotin ligase BioID2, and a HA tag.

Depositor

Yuxuan Guo

Article

Guo et al Acta Pharmacol Sin. 2024 Jul 23. doi: 10

Insert

BioID-HA (Actn2 Synthetic)

Use

AAV

Tags

HA

Expression

Mutation

Promoter

Available since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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HA-GFP1-10-actin-pcDNA4-TO

Plasmid

#118370

Purpose

Bimolecular Fluorescence Complimentation (BiFC) assay. Mammalian expression vector to generate stable, inducible cell line expressing N-terminally tagged HA-GFP1-10-actin.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

actin beta (ACTB Human)

Use

Tags

HA-GFP1-10

Expression

Mammalian

Mutation

Promoter

CMV/TO

Available since

May 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+_LN-HA-AGO2-5xA

Plasmid

#92010

Purpose

Expresses Lambda-N-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Tags

Lambda-N-HA

Expression

Mammalian

Mutation

S824A, S828A, T830A, S831A, S834A (5xA)

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+_LN-HA-AGO2-5xE

Plasmid

#92011

Purpose

Expresses Lambda-N-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Tags

Lambda-N-HA

Expression

Mammalian

Mutation

S824E, S828E, T830E, S831E, S834E (5xE)

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsAGO2-K663AL697Y_AC

Plasmid

#148427

Purpose

Mammalian Expression of HsAGO2-K663AL697Y

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsAGO2-K663AL697Y (AGO2 Human)

Use

Tags

Expression

Mammalian

Mutation

three silent mutations and the sequence differs i…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsAGO2-P593GA623FK663AL697Y_AC

Plasmid

#148428

Purpose

Mammalian Expression of HsAGO2-P593GA623FK663AL697Y

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsAGO2-P593GA623FK663AL697Y (AGO2 Human)

Use

Tags

Expression

Mammalian

Mutation

three silent mutations and the sequence differs i…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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Gal4-HA-HIF1α(530-652)

Plasmid

#24887

Depositor

William Kaelin

Article

Ivan et al Science. 2001 Apr 20. 292(5516):464-8.

Insert

hypoxia inducible factor1alpha (HIF1A Human)

Use

Tags

Gal4dbd and HA-tag

Expression

Mammalian

Mutation

partial HIF1alpha cDNA (encoding aa 530-652)fused…

Promoter

Available since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

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p(HA)HIF1alpha(401delta603)R27G

Plasmid

#52215

Purpose

mammalian expression of HA tagged HIF1alpha with a deletion of the N-terminal activation domain and R27G mutation

Depositor

Eric Huang

Article

Koshiji et al EMBO J. 2004 May 5;23(9):1949-56. Epub 2

Insert

HIF1alpha (401delta603) R27G (HIF1A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

aa 401-603 deleted, removes the oxygen-dependent …

Promoter

CMV

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
GFP Cav2.2 HA D122A pcDNA3

Plasmid

#206120

Purpose

expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

Depositor

Annette Dolphin

Article

Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

D122A

Promoter

CMV

Available since

Nov. 22, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 HA E1658A (EIVA) pcDNA3

Plasmid

#206114

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658A mutation in the P loop of Domain IV which reduces trafficking to the cell surface

Depositor

Annette Dolphin

Article

Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

E1658A

Promoter

CMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.1 HA EIA (E320A) pcDNA3

Plasmid

#206124

Purpose

expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain I to abolish divalent cation binding

Depositor

Annette Dolphin

Article

Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

Insert

cacna1a (Cacna1a Rat)

Use

Tags

Expression

Mammalian

Mutation

E320A

Promoter

CMV

Available since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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An R57A, R59A HA CAAX pMT2

Plasmid

#206126

Purpose

Cav2.1 N-terminus (amino acids 1-100) with R57A and R59A mutations that prevent dominant-negative suppression of Cav2.1 currents and a C-terminal CAAX (last 10 aa of H.Ras) motif and a HA tag

Depositor

Annette Dolphin

Article

Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

Insert

cacna1a (Cacna1a Rat)

Use

Tags

Expression

Mammalian

Mutation

R57A, R59A

Promoter

Ad MLP/TPL/SV40

Available since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 HA EIVA (E1658A) pCAGGS

Plasmid

#206082

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain IV which reduces trafficking to the cell surface

Depositor

Annette Dolphin

Article

Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

E1658A

Promoter

CMV/B-actin

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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