-
Plasmid#80362PurposePmex-5:GFP::H2B::PEST::set-6 3'UTR reporter construct used for microinjectionDepositorInsertPmex-5:GFP::H2B::PEST::set-6 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-ets4
Plasmid#80355PurposePmex-5:GFP::H2B::PEST::ets-4 3'UTR reporter construct used for microinjectionDepositorInsertPmex-5:GFP::H2B::PEST::ets-4 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-mex3
Plasmid#80361PurposePmex-5:GFP::H2B::PEST::mex-3 3'UTR reporter construct used for microinjectionDepositorInsertPmex-5:GFP::H2B::PEST::mex-3 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-mbk2
Plasmid#80360PurposePmex-5:GFP::H2B::PEST::mbk-2 3'UTR reporter construct used for microinjectionDepositorInsertPmex-5:GFP::H2B::PEST::mbk-2 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIK137
Plasmid#65632PurposeExpresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter and contains a C. briggsae unc-119 rescuing fragmentDepositorInsertPeft-3::gfp::h2b::tbb-2 3'UTR, C. briggsae unc-119 rescue fragment
UseTagsExpressionWormMutationPromoterAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAP1071-2
Plasmid#99502Purpose3XFlag::meGFP with 2 introns::Ollas::H2B::tbb-2 3utr::linker::TEVDepositorInsert3XFlag::meGFP with 2 introns::Ollas::H2B::tbb-2 3’utr::linker::TEV
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
JPF0446
Plasmid#124022PurposeBxBI Destination vector with H2B::GFP expressed from CAG promoterDepositorInsertBXBI_pCAG-H2B::msfGFP-bghpA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
iChr2-Control-Mosaic (SO274)
Plasmid#99751PurposeSecond generation Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different chromatin localized fluorescent proteinsDepositorInsertHIs-H2B-Cherry, H2B-EGFP, HA-H2B-Cerulean
UseCre/Lox and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
iChr2-Notch Mosaic (SO250)
Plasmid#99752PurposeSecond generation Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different chromatin localized fluorescent proteins and Notch signalling genesDepositorInsertHIs-H2B-Cherry, H2B-EGFP-V5, HA-H2B-Cerulean, DN-Maml1, NICD-PEST
UseCre/Lox and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBOB-synP-HTB
Plasmid#30195DepositorInserthistone-GFP-2A-TVA receptor-2A-rabies glycoprotein
UseLentiviralTagseGFPExpressionMammalianMutationPromoterAvailable sinceApril 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
-
Ubiquiton Human Kit
Plasmid Kit#1000000244PurposeThe Ubiquiton system allows the inducible, linkage-specific polyubiquitylation of a protein of interest, accommodating for linear (M1-), K48-, and K63-linkage.DepositorAvailable sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM11
Plasmid#135483PurposeAgrobacterium T-DNA with SpunH2Bpr driving hph-GFPDepositorInsertSpunH2Bpr hph-GFP ADH1t
UseTagsGFPExpressionBacterial and YeastMutationPromoterSpunH2BprAvailable sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
PAX6 TALEN R
Plasmid#105525PurposeKnock in H2B-GFP into the human PAX6 locusDepositorInsertPax6 TALEN R
UseTALENTags3x FLAG and NLSExpressionMammalianMutationPromoterCMVAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
PAX6 TALEN L
Plasmid#109034PurposeKnock in H2B-GFP into the human PAX6 locusDepositorInsertPax6 TALEN L
UseTALENTags3x FLAG and NLSExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCFJ914
Plasmid#44489DepositorInsertMinimal Mos1 [Peft-3 GFP H2B tbb-2 UTR, NeoR]
UseGateway expression cloneTagsExpressionMutationPromoterAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
C. elegans miniMos transgenesis kit
Plasmid Kit#1000000031Purpose29 plasmid kit contains reagents for generating transgenic nematodes, including C. elegans and C. briggsae, using miniMos transposon. Selection with unc-119 or NeoR, PuroR.DepositorAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Tm2AG
Plasmid#26773DepositorInsertTdTomato-2A-H2BGFP-SV40pA
UseTagsExpressionMammalianMutationmutation in 2A peptide that renders it non-functi…PromoterAvailable sinceMarch 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
Tol2 Gateway compatible toolbox for the study of the nervous system and neurodegenerative disease
Plasmid Kit#1000000087PurposeTol2 Zebrafish toolkit: 4 promoters, 6 fluorophores, MultiSite Gateway (Invitrogen) compatible and cloning sites. Production transgenic zebrafish for neurodegenerative disordersDepositorAvailable sinceOct. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMCh2007_pLenti_Syn_mChe-cdc42E7
Plasmid#118620Purposeexpression of mChe-tagged cdc42E7 under pSyn promoter for primary neuronsDepositorInsertcdc42E7 (Cdc42 Mouse)
UseLentiviralTagsmCherryExpressionMammalianMutationchanged cagtctg to ggcataa (667 - 673 nt in 3…Promotersynapsin I (rat)Available sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only