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Plasmid#223001PurposeHDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_armDepositorInsertPE7
UseHdr donorTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationPromoterEF1αAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_hPGK1_EGFP_Donor
Plasmid#178088PurposeHDR donor plasmid to introduce the EGFP cassette to AAVS1 using AAVS1 T2 sgRNA.DepositorInsertEGFP
UseCRISPRTagsExpressionMammalianMutationPromoterHuman PGK1Available sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDonor_sU6
Plasmid#69351PurposesgRNA scaffold and synthetic U6 promoterDepositorInsertsU6 promoter and sgRNA scaffold flanked by BbsI sites
UseCRISPRTagsExpressionMutationPromotersynthetic U6 promoterAvailable sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
PEmax_AAVS1_knockin_HDR_donor
Plasmid#223000PurposeHDR donor plasmid for PEmax knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PEmax-IRES2-EGFP-WPRE-βglobinpA-right_homology_armDepositorInsertPEmax
UseHdr donorTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationPromoterEF1αAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMPRAdonor2
Plasmid#49353PurposeminP-luc2 donor plasmid for MPRA library constructionDepositorInsertluc2
UseLuciferaseTagsExpressionMutationPromoterminPAvailable sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
AY42_pS.Donor.R5
Plasmid#100291PurposeCCR5-targeting plasmidDepositorInsert18 bp RFLP (MluI+KspAI)
UseCRISPR and Synthetic Biology; Human targetingTagsExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDonor_hU6
Plasmid#69312PurposesgRNA scaffold and human U6 promoterDepositorInserthU6 promoter and sgRNA scaffold flanked by BbsI sites
UseCRISPRTagsExpressionMutationPromoterhuman U6 promoterAvailable sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_hPGK1_EBFP_Donor
Plasmid#178089PurposeHDR donor plasmid to introduce the EBFP (T65S/F145Y) cassette to AAVS1 using AAVS1 T2 sgRNA.DepositorInsertEBFP (T65S/F145Y)
UseCRISPRTagsExpressionMammalianMutationPromoterHuman PGK1Available sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
AD59_pEP.DonorCLYBL.TS
Plasmid#199227PurposeDonor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locusDepositorInsertExpression unit for PuroR.T2A.EGFP selectable and reporter markers
UseGene targeting donor plasmidTagsExpressionMammalianMutationPromoterHuman PGK1 gene promoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDonor_mU6
Plasmid#69350PurposesgRNA scaffold and mouse U6 promoterDepositorInsertmU6 promoter and sgRNA scaffold flanked by BbsI sites
UseCRISPRTagsExpressionMutationPromotermU6 promoterAvailable sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_plasmid_donor_RD
Plasmid#86551PurposeHomology-directed repair (HDR) donor plasmid with homology arms to ATP1A1 to introduce the Q118R and N129D (RD) mutations conferring cellular resistance to ouabainDepositorInsertATP1A1 (ATP1A1 Human)
UseCRISPR and TALEN; Co-selection via hdr using ouab…TagsExpressionMammalianMutationQ118R, N129DPromoterAvailable sinceMarch 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDonor_AcCAST_kanR
Plasmid#127925PurposeKanamycin resistance donor for AcCASTDepositorInsertKanR
UseTagsExpressionMutationPromoterAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
AZ64_pE.DonorCLYBL.TS
Plasmid#199228PurposeDonor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locusDepositorInsertExpression unit for EGFP reporter
UseGene targeting donor plasmidTagsExpressionMammalianMutationPromoterHuman PGK1 gene promoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
EZH2_N_FS_v2_Donor
Plasmid#79901PurposeN-terminal tagging of human EZH2 with 3xFLAG_Twin_StrepDepositorTypeEmpty backboneUseCRISPR and TALENTags3xFLAG_Twin_StrepExpressionMutationPromoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
BB44_pmc.DonorR5.TS
Plasmid#199223PurposeDonor plasmid with CCR5 target sites for ITPN or HMEJ knock-in at the human CCR5 safe harbour locusDepositorInsertExpression unit for mCherry - monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)
UseGene targeting donor plasmidTagsExpressionMammalianMutationPromoterHuman PGK1 gene promoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
AV09_pDonor.EP.S1.TS
Plasmid#100297PurposeAAVS1-targeting expression plasmidDepositorInsertPuroR.T2A.EGFP expression unit
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_T804N_hPGK1_EBFP_Donor
Plasmid#187455PurposeHDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the EBFP cassette to ATP1A1 intron 17.DepositorInsertATP1A1-T804N EBFP donor (ATP1A1 Human)
UseTagsExpressionMammalianMutationPromoterhPGK1Available sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMPRAdonor1
Plasmid#49352PurposePromoterless luc2 donor plasmid for MPRA library constructionDepositorInsertluc2
UseLuciferaseTagsExpressionMutationPromoterNoneAvailable sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
sgDonor_lentiGuide
Plasmid#184168PurposeLentiGuide-puro vector expressing the sgRNA necessary for linearization of the donor cassette plasmid.DepositorInsertsgDonor
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMPRAdonor3
Plasmid#49354PurposepSV40mod-luc2 donor plasmid for MPRA library constructionDepositorInsertluc2
UseLuciferaseTagsExpressionMutationPromoterSV40modAvailable sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only