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Addgene
Showing: 181 - 200 of 8063 results

  1. pLSU1/t35s[TMV]:mNeonGreen-I{PIV-390}:NosT

    Plasmid
    #212168
    Purpose
    This binary vector expresses mNeonGreen containing the PIV intron (390bp into the gene) with the truncated 35sCAMV promoter and the tomato mosaic virus UTR
    Depositor
    Wayne Curtis
    Insert
    Tomato codon optimized version of mNeonGreen-I{PIV-390}
    Use
    Tags
    Expression
    Plant
    Mutation
    mNeonGreen codon-optimized for tomato and PIV int…
    Promoter
    truncated 35sCaMV
    Availability
    Academic Institutions and Nonprofits only
    212168-plasmid-map-sequence-id-422679

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  2. pGEX6P1 METTL3

    Plasmid
    #200731
    Purpose
    Bacterial expression of GST-Tagged codon optimized human METTL3
    Depositor
    Claudio Alarcón
    Insert
    METTL3 (METTL3 Human)
    Use
    Tags
    GST from backbone plasmid
    Expression
    Bacterial
    Mutation
    Codon optimized for expression in E. coli
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    200731-plasmid-map-sequence-id-401808

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  3. JDS246

    Plasmid
    #43861
    Purpose
    Expresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promoters
    Depositor
    Keith Joung
    Insert
    mammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag
    Use
    CRISPR
    Tags
    3X FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    43861-plasmid-map-sequence-id-59200

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  4. pFastBac_Dual_SNF2L-opt_p48

    Plasmid
    #102243
    Purpose
    Expression of human codon optimized human SNF2L and pRbAp48 from pFastBac dual
    Depositor
    Joe Landry
    Inserts
    SNF2L (SMARCA1 Human)
    pRbAp48 (RBBP4 Human)
    Use
    Tags
    Expression
    Insect
    Mutation
    codon optimized for expression
    Promoter
    Polyhedrin and p10
    Availability
    Industry, Academic Institutions, and Nonprofits
    102243-plasmid-map-sequence-id-197769

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  5. pAH-CTX1-rhadCas9

    Plasmid
    #129391
    Purpose
    Derived from pAH-CTX1-rha. The Burkholderia cenocepacia codon-optimized dCas9 cloned downstream of PrhaBAD. Hence, dCas9 is controlled by the rhamnose-inducible promoter system.
    Depositor
    Silvia Cardona
    Article
    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021
    Insert
    Burkholderia cenocepacia codon-optimized dCas9
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Entire dCas9 codon optimized for the GC-rich B. c…
    Promoter
    PrhaBAD
    Availability
    Academic Institutions and Nonprofits only
    129391-plasmid-map-sequence-id-273754

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  8. pCRISPR-aBEST

    Plasmid
    #131464
    Purpose
    Expression of a Streptomyces codon optimized Cas9n-tadA fusion protein in pGM1190. A to G base editor for actinomycetes
    Depositor
    Tilmann Weber
    Article
    Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116
    Inserts
    Streptomyces codon optimized spCas9n (D10A)
    streptomyces codon optimized tadA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    tipA
    Availability
    Academic Institutions and Nonprofits only
    131464-plasmid-map-sequence-id-261686

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  10. pBAD-bHs

    Plasmid
    #134259
    Purpose
    For L-arabinose inducible bacterial expression of S. typhimurium codon-optimized hyaluronidase (Streptomyces koganeinsis UniProt A0A0U2E2) with N-terminal 6x His tag
    Depositor
    Edwin Manuel
    Article
    Ebelt et al Mol Cancer Ther. 2019 Nov 6. pii: 1535-7
    Insert
    Hyaluronidase from Streptomyces koganeinsis codon-optimized for Salmonella typhimurium
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    codon optimized for Salmonella typhimurium
    Promoter
    AraC
    Availability
    Academic Institutions and Nonprofits only
    134259-plasmid-map-sequence-id-264461

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  12. pORFE0005

    Plasmid
    #112073
    Purpose
    AtDeadCAS9 D10A H840A without stop codon module CDS1ns
    Depositor
    Christian Chevalier
    Insert
    AtDeadCAS9 D10A H840A without stop codon
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Plant codon optimized dCas9 D10A S. pyogenes
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    112073-plasmid-map-sequence-id-217642

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  14. pmCellFree_KA1_SARS-COV-2_ORF7a

    Plasmid
    #169393
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF7a with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF7a (ORF7a SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Availability
    Academic Institutions and Nonprofits only
    169393-plasmid-map-sequence-id-336506

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  15. pLSU1/t35s[TMV]:mNeonGreen-I{AGO-198}:NosT

    Plasmid
    #212169
    Purpose
    This binary vector expresses mNeonGreen containing the Argonaut (AGO) intron (198bp into the gene) with the truncated 35sCAMV promoter and the tomato mosaic virus UTR
    Depositor
    Wayne Curtis
    Insert
    Tomato codon optimized version of mNeonGreen-I{AGO-198}
    Use
    Tags
    Expression
    Plant
    Mutation
    mNeonGreen codon-optimized for tomato and AGO int…
    Promoter
    truncated 35sCaMV
    Availability
    Academic Institutions and Nonprofits only
    212169-plasmid-map-sequence-id-422695

    Enlarge

  16. pLSU1/t35s[TMV]:mNeonGreen-I{PDK-198}:NosT

    Plasmid
    #212170
    Purpose
    This binary vector expresses mNeonGreen containing the castor bean catalase (PDK) intron (198bp into the gene) with the truncated 35sCAMV promoter and the tomato mosaic virus UTR
    Depositor
    Wayne Curtis
    Insert
    Tomato codon optimized version of mNeonGreen-I{PDK-198}
    Use
    Tags
    Expression
    Plant
    Mutation
    mNeonGreen codon-optimized for tomato and PDK int…
    Promoter
    truncated 35sCaMV
    Availability
    Academic Institutions and Nonprofits only
    212170-plasmid-map-sequence-id-422699

    Enlarge

  20. pET-HIS-Sangamo

    Plasmid
    #40786
    Purpose
    for e. coli expression, Golden Gate Compatible TALEN incorporated with N Terminal 6xHIS Tag, Codon Optimized FokI
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Nuclease
    Use
    TALEN
    Tags
    N Terminal 6xHIS
    Expression
    Bacterial
    Mutation
    +63 C Terminus, Codon Optimized FokI Endonuclease
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    40786-plasmid-map-sequence-id-68901

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Showing: 181 - 200 of 8063 results