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Showing: 181 - 200 of 3034 results

  5. pcDNA3.1-N-Myc_DW164AA + DED/AAA USP7

    Plasmid
    #131256
    Purpose
    Mammalian expression of N-terminally Myc-tagged USP7, with mutations in the TRAF-like domain (DW164AA) and UBL2 (DE758AA_D764A)
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d
    Insert
    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Contains point mutations that convert USP7 aspart…
    Promoter
    Available since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    131256-plasmid-map-sequence-id-267711

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  6. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAAA)

    Plasmid
    #137860
    Purpose
    Monomeric TagBFP2 and iCre-ERT2 recombinase (PAAA N-terminus)
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCreERT2
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    137860-plasmid-map-sequence-id-274446

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  10. 1357 pcDNA3 flag FKHR AAA delta DB

    Plasmid
    #10696
    Depositor
    William Sellers
    Insert
    FKHR AAA deltaDB (FOXO1 Human)
    Use
    Tags
    flag
    Expression
    Mammalian
    Mutation
    T24A S256A S319A , deletion in aa 208-220
    Promoter
    Available since
    April 4, 2006
    Availability
    Academic Institutions and Nonprofits only

  11. (194) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4,K852A)

    Plasmid
    #160742
    Purpose
    Expresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to the EPEA nanobody. A different nanobody can be inserted by using Sgfl + Sgsl restriction enzymes.
    Depositor
    Christina Woo
    Article
    Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10
    Insert
    HA-nEPEA-(EAAAK)4-OGT(4,K852A)
    Use
    Tags
    HA-Tag
    Expression
    Mammalian
    Mutation
    Promoter
    T7/CMV
    Available since
    May 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160742-plasmid-map-sequence-id-332924

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  17. 1358 pBabe puroL FKHR AAA delta DB

    Plasmid
    #10697
    Depositor
    William Sellers
    Insert
    FKHR AAA deltaDB (FOXO1 Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    T24A S256A S319A , deletion in aa 208-220
    Promoter
    Available since
    April 4, 2006
    Availability
    Academic Institutions and Nonprofits only

  18. Cenp-B DBD INCENP TSS/AAA mCherry

    Plasmid
    #45234
    Purpose
    Target INCENP (with TSS mutated to AAA) to centromeres via CENP-B DNA binding domain (DBD)
    Depositor
    Michael Lampson
    Article
    Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.
    Insert
    Cenp-B DBD, INCENP-ΔCEN with AAA mutation, mCherry
    Use
    Tags
    CENP-B DBD, DNA binding domain of CENP-B and mChe…
    Expression
    Mammalian
    Mutation
    INCENP C-terminal TSS mutated to AAA
    Promoter
    t7
    Available since
    Sept. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    45234-plasmid-map-sequence-id-84485

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  19. pCEP-Puro-TetOn-L1RP-ORF1_StammerAAA_Halo-GFPai

    Plasmid
    #202571
    Purpose
    Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the M91A, E92A, and L93A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    LINE-1
    Use
    Tags
    GFP-AI retrotransposition reporter and HaloTag7 o…
    Expression
    Mammalian
    Mutation
    Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…
    Promoter
    Available since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    202571-plasmid-map-sequence-id-429226

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Showing: 181 - 200 of 3034 results