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Plasmid#82451PurposeChain B of the habu snake venom FIX/FX binding protein with C-terminal biotin-tag. Cloned into pIRESneo3 (Ampicillin) via 5' NheI and 3' BamHI; IRES vector conferring puromycin resistance.DepositorInsertrFIXX-bp chain B
UseTagsBiotinExpressionBacterial and MammalianMutationPromoterT7 RNA polymerase promoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS4
Plasmid#55751PurposeProduces one half (alpha) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.DepositorInsertKanR
UseTagsExpressionBacterialMutationResidues 1-59 of KanRPromoterT7-lacAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCS5
Plasmid#55750PurposeProduces one half (beta) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.DepositorInsertKanR
UseTagsExpressionBacterialMutationResidues 59-264 of KanRPromoterT7-lacAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
p2,0_3M5M_luciferase
Plasmid#135475PurposeEncodes Marburg virus minigenome with luciferase reporter gene under control of a T7 RNA polymerase promoterDepositorInsertMarburg virus minigenome, luciferase reporter
UseTagsExpressionMammalianMutationNonePromoterT7Available sinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2,0_3M5M_eGFP
Plasmid#135474PurposeEncodes Marburg virus minigenome with eGFP reporter gene under control of a T7 RNA polymerase promoterDepositorInsertMarburg virus minigenome, eGFP reporter
UseTagsExpressionMammalianMutationNonePromoterT7Available sinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJP_Azul01
Plasmid#219670PurposeBlue light inducible systemDepositorInsertssimilar to T7 RNA polymerase (core)
similar to YF1-fixJ
UseSynthetic BiologyTagsExpressionMutationPromoterLacIq35 and pJ23105Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Rluc-A114-N40
Plasmid#50561PurposeTemplate DNA for in vitro transcription using T7 polymeraseDepositorInsertLuciferase-A114-N40
UseCloning vectorTagsExpressionMutationPromoterlacAvailable sinceFeb. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCfB8589
Plasmid#175221PurposeXenopus oocyte expression vector containing YMR253C. Contains T7 RNA polymerase binding site, and adds polyA tail to YMR253C. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYMR253C
UseMrna expression, injection into xenopus oocytesTagsPolyAExpressionMutationPromoterT7Available sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB8707
Plasmid#175222PurposeXenopus oocyte expression vector containing YNL095C. Contains T7 RNA polymerase binding site, and adds polyA tail to YNL095C. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYNL095C
UseMrna expression, injection into xenopus oocytesTagsPolyAExpressionMutationPromoterT7Available sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB8728
Plasmid#175223PurposeXenopus oocyte expression vector containing YOL060C. Contains T7 RNA polymerase binding site, and adds polyA tail to YOL060C. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYOL060C
UseMrna expression, injection into xenopus oocytesTagsPolyAExpressionMutationPromoterT7Available sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB8709
Plasmid#175224PurposeXenopus oocyte expression vector containing YOL092W. Contains T7 RNA polymerase binding site, and adds polyA tail to YOL092W. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYOL092W (CEN.PK strain)
UseMrna expression, injection into xenopus oocytesTagsPolyAExpressionMutationPromoterT7Available sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB8711
Plasmid#175225PurposeXenopus oocyte expression vector containing YOR092W. Contains T7 RNA polymerase binding site, and adds polyA tail to YOR092W. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYOR092W
UseMrna expression, injection into xenopus oocytesTagsPolyAExpressionMutationPromoterT7Available sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB8713
Plasmid#175226PurposeXenopus oocyte expression vector containing YOR271C. Contains T7 RNA polymerase binding site, and adds polyA tail to YOR271C. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYOR271C
UseMrna expression, injection into xenopus oocytesTagsPolyAExpressionMutationPromoterT7Available sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB8590
Plasmid#175227PurposeXenopus oocyte expression vector containing YOR307C. Contains T7 RNA polymerase binding site, and adds polyA tail to YOR307C. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYOR307C
UseMrna expression, injection into xenopus oocytesTagsPolyAExpressionMutationPromoterT7Available sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB8738
Plasmid#175228PurposeXenopus oocyte expression vector containing YPL189W. Contains T7 RNA polymerase binding site, and adds polyA tail to YPL189W. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYPL189W
UseMrna expression, injection into xenopus oocytesTagsPolyAExpressionMutationPromoterT7Available sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB8591
Plasmid#175229PurposeXenopus oocyte expression vector containing YPL244C. Contains T7 RNA polymerase binding site, and adds polyA tail to YPL244C. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYPL244C
UseMrna expression, injection into xenopus oocytesTagsPolyAExpressionMutationPromoterT7Available sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB8592
Plasmid#175230PurposeXenopus oocyte expression vector containing YPL264C. Contains T7 RNA polymerase binding site, and adds polyA tail to YPL264C. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYPL264C
UseMrna expression, injection into xenopus oocytesTagsPolyAExpressionMutationPromoterT7Available sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB8715
Plasmid#175231PurposeXenopus oocyte expression vector containing YPR201W. Contains T7 RNA polymerase binding site, and adds polyA tail to YPR201W. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYPR201W
UseMrna expression, injection into xenopus oocytesTagsPolyAExpressionMutationPromoterT7Available sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCFB8456
Plasmid#175208PurposeXenopus oocyte expression vector containing YEL004W. Contains T7 RNA polymerase binding site, and adds polyA tail to YEL004W. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYEL004W
UseMrna expression, injection into xenopus oocytesTagsPolyAExpressionMutationPromoterT7Available sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCFB8457
Plasmid#175209PurposeXenopus oocyte expression vector containing YER039C. Contains T7 RNA polymerase binding site, and adds polyA tail to YER039C. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYER039C
UseMrna expression, injection into xenopus oocytesTagsPolyAExpressionMutationPromoterT7Available sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only