-
Plasmid#99603PurposePlasmid containing the parB-m(sf)gfp fusionDepositorInsertparBp
UseSynthetic BiologyTagsm(sf)gfpExpressionMutationPromoterPczcDAvailable sinceSept. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJH375
Plasmid#86841Purposemamalian expression of anti-CRISPR protein AcrIIA3DepositorInsertacrIIA3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMK11-02
Plasmid#99606PurposePlasmid containing parBp(mut)-mKate2 fusionDepositorInsertparBp(mut)-mKate2
UseSynthetic BiologyTagsmKate2ExpressionMutationinternal parS-site mutatedPromoterPczcDAvailable sinceSept. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCSW24
Plasmid#86837Purposebacterial expression of anti-CRISPR protein AcrIIA3DepositorInsertacrIIA3
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceOct. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS303-pGAL-HA-SrtAstrep (pKS78)
Plasmid#50029PurposeGalactose-inducible expression of cytosolic S. pyogenes SrtA in yeastDepositorInsertHA-SrtA(strep)
UseTagsHAExpressionYeastMutationPromoterpGALAvailable sinceJan. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS303-pGAL-HA-TMSec66-SrtA (pKS105)
Plasmid#50033PurposeGalactose-inducible expression of ER-membrane S. pyogenes SrtA in yeastDepositorInsertSrtA(strep)
UseTagsHAExpressionYeastMutationPromoterpGALAvailable sinceJan. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
A9QH69
Plasmid#163216PurposeInducible expression of UniProt identifier A9QH69DepositorInsertA9QH69
UseTags10xHis and T7ExpressionBacterialMutationPromoterT7Available sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDAC565
Plasmid#195242PurposeMammalian expression of Csm-GFP complex (RNase mut) from separate promoters (Used for RNA imaging)DepositorInsertFLAG-NLS-Csm1; FLAG-NLS-Csm2, FLAG-NLS-Csm3(RNase mut)-GFP; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDAC439
Plasmid#195237PurposeMammalian expression of Csm complex from single promoter; RFP backbone (Used for RNA knockdown)DepositorInsertFLAG-NLS-Csm5-2A-FLAG-NLS-Csm4-2A-FLAG-NLS-Csm3-2A-FLAG-NLS-Csm2; RFP-2A-FLAG-NLS-Cas6-2A-FLAG-NLS-Csm1; crRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-J6-dCas9-24XGP41-J9
Plasmid#202006PurposeMoClo level 0 dCas9-24XGP41 coding sequence moduleDepositorInsertdCas9-24XGP41
UseSynthetic Biology; Moclo cloning vectorTagsGS linkersExpressionMutationPromoterAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTdTomato-L5
Plasmid#140994PurposetdTomato, StrepR, L5int, attP to create RFP mycobacteriaDepositorInserttdTomato
UseTagsExpressionBacterialMutationPromoterhsp60Available sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL0_12 [pAMβ1 ORI]
Plasmid#198926PurposeLevel 0 partDepositorInsertpAMβ1 ORI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHO4d-Cas9
Plasmid#67881PurposeCas9nlsHis6 Bacterial Expression constructDepositorInsertCas9
UseCRISPRTagsHis6 and T7 epitopeExpressionBacterialMutationPromoterT7Available sinceSept. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKIR1.1
Plasmid#85758PurposeCRISPR/Cas9 in Arabidopsis with seed a fluorescent reporterDepositorInserthuman-codon-optimized SpCas9
UseCRISPRTagsFLAGExpressionPlantMutationPromoterAtRPS5AAvailable sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMC-J6-dCas9-10XGP41-J9
Plasmid#202005PurposeMoClo level 0 dCas9-10XGP41 coding sequence moduleDepositorInsertdCas9-10XGP41
UseSynthetic Biology; Moclo cloning vectorTagsGS linkersExpressionMutationPromoterAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDAC569
Plasmid#195241PurposeMammalian expression of Csm complex (RNase mut) from separate promoters; GFP backbone (Used for RNA binding/tethering)DepositorInsertFLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3(RNase mut); FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDL278_P23-DsRed-Express2
Plasmid#121505PurposeE. coli-streptococcal shuttle vector that expresses rfpDepositorInsertDsRed-Express2
UseE. coli streptococcal shuttle vectorTagsExpressionMutationPromoterP23Available sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKI1.1R
Plasmid#85808PurposeCRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.DepositorInserthuman-codon-optimized SpCas9
UseCRISPRTagsFLAG and NLSExpressionPlantMutationPromoterAtRPS5AAvailable sinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-nCas9n-A71
Plasmid#125644Purposemicro-injection of poly-adenylated nCas9n RNADepositorInsertnCas9n
UseRna in vitro transcription vectorTagsExpressionMutationPromoterT3Available sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPM_Pveg-sfgfp
Plasmid#121503PurposeIntegration vector that expresses gfp in S. mutansDepositorInsertSuperfolder GFP
UseS. mutans integration vector, integrates at the m…TagsExpressionMutationPromoterPvegAvailable sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only