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Plasmid#162895PurposeStandard Gateway entry clone encoding mOrange fluorescent proteinDepositorInsertmonomeric Orange fluorescent protein
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
mOrange-H2B-6
Plasmid#57942PurposeLocalization: Nucleus/Histones, Excitation: 548, Emission: 562DepositorInsertH2B (H2BC11 Human)
UseTagsmOrangeExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
mOrange2-H2B-6
Plasmid#57962PurposeLocalization: Nucleus/Histones, Excitation: 549, Emission: 565DepositorInsertH2B (H2BC11 Human)
UseTagsmOrange2ExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFUGW ORANGE Gria1-GFP KI _ mCherry-KASH
Plasmid#131507PurposeEndogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)DepositorUseLentiviralTagsExpressionMammalianMutationPromoterhUBCAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRAN473
Plasmid#120813PurposeE. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt), multiple cloning site for fusion to N-terminus of target proteinDepositorInsertred fluorescent protein (mCherry), codon-optimized for C. difficile, multiple-cloning site for protein fusion
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFS_0235-pET30-Fusicatenibacter_saccharivorans_ARRAY2_FaqI
Plasmid#116954PurposeExpresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0160-pET30-Fusicatenibacter_saccharivorans_ARRAY2
Plasmid#116953PurposeExpresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with classic acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
membrane-mCherry-SpVasa-C3
Plasmid#185587Purposeto recruit Vasa-C3 to membraneDepositorInsertVasa-C3
UseIn vitro transcription (mrna synthesis)TagsmCherry and membrane targeting sequence from Neur…ExpressionMutationC3 mutantPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET11a-hRanGAP1
Plasmid#50941Purposeexpression of full length untagged human RanGAP1 in E.coliDepositorInsertRanGTPase activating protein 1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
mOrange2-Cx43-7
Plasmid#57951PurposeLocalization: Gap Junctions, Excitation: 549, Emission: 565DepositorInsertCx43
UseTagsmOrange2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX3x-hRanBP2-(IR1+M)
Plasmid#53140PurposeE.coli expression of GST tagged human RanBP2 fragment with SUMO ligase activityDepositorInsertRanBP2 fragment (RANBP2 Human)
UseTagsGSTExpressionBacterialMutationfragment (amino acids 2631-2711)PromotertacAvailable sinceMay 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSTAR-mOrange2-sfGFP
Plasmid#115693PurposeGeneration of CD-tagged libraries with an mOrange2-sfGFP Mammalian Cell-Optimized Fluorescent TimerDepositorInsertSA-mOrange2-sfGFP-SD
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-lssmOrange
Plasmid#179795PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertlss mOrange
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-sgRANGAP.C1-CAG-Cas9-T2A-mCherry-P2A-Puro
Plasmid#216249PurposeExpress Cas9 with CAG promoter to improve the expression in hES cells and sgRANGAP.C1 to tag endogenous RANGAP1 C-terminusDepositorInsertCas9 and sgRANGAP.C1 (RANGAP1 Human)
UseCRISPR; Endogenous taggingTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoPSmOrange-Kan
Plasmid#44951PurposeYeast tagging vector to create gene fusion with yeast optimized photoconvertible mOrange with Kan selectionDepositorInsertyoPSmOrange
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoPSmOrange-SpHis5
Plasmid#44856PurposeYeast tagging vector to create gene fusion with yeast optimized photoconvertible mOrange with SpHis5 selectionDepositorInsertyoPSmOrange
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
mOrange2-Tubulin-6
Plasmid#57974PurposeLocalization: Microtubules, Excitation: 549, Emission: 565DepositorInsertTubulin (TUBA1B Human)
UseTagsmOrange2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_CUP1-1
Plasmid#166086PurposePlasmid for constituive spCas9 and tet-inducible CUP1-1 targeting sgRNA expression for double stranded break formation in yeastDepositorInsertCUP1-1 (CUP1-1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
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mRFP1-1
Plasmid#63816PurposeA member of an RBS library for mRFP1 with different translation ratesDepositorInsertmRFP1
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterJ23100Available sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only