Addgene

We narrowed to 5,231 results for: Pet2;

Showing: 181 - 200 of 5231 results

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AoC_pET29b

Plasmid

#215402

Purpose

Expression construct for AoC gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

AoC

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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M-NylC_pET28a

Plasmid

#215409

Purpose

Expression construct for M-NylC gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

M-NylC

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET28b_TypeIC_acrIF2_invivo

Plasmid

#196402

Purpose

expresses acrIF2 protein targeting CRISPR Cascade Type IC for in vivo assay

Depositor

David Taylor

Article

O'Brien et al Mol Cell. 2023 Mar 2;83(5):746-758.e5. d

Insert

anti-CRISPR IF2 protein (orf30 P. aeruginosa)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET28b_TypeIC_acrIC4_invivo

Plasmid

#196403

Purpose

expresses acrIC4 protein targeting CRISPR Cascade Type IC for in vivo assay

Depositor

David Taylor

Article

O'Brien et al Mol Cell. 2023 Mar 2;83(5):746-758.e5. d

Insert

anti-CRISPR IC4 protein

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET24a_thyA_R166Q_untagged

Plasmid

#191307

Purpose

Bacterial expression of E. coli thyA (thymidylate synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

thyA

Use

Tags

Expression

Bacterial

Mutation

R166Q TYMS

Promoter

T7

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pET24a_thyA_Q33S_untagged

Plasmid

#191308

Purpose

Bacterial expression of E. coli thyA (thymidylate synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

thyA

Use

Tags

Expression

Bacterial

Mutation

Q33S TYMS

Promoter

T7

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pET24a_thyA_R127A_untagged

Plasmid

#191309

Purpose

Bacterial expression of E. coli thyA (thymidylate synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

thyA

Use

Tags

Expression

Bacterial

Mutation

R127A TYMS

Promoter

T7

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pET24a_thyA_WT_untagged

Plasmid

#191306

Purpose

Bacterial expression of E. coli thyA (thymidylate synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

thyA

Use

Tags

Expression

Bacterial

Mutation

WT TYMS

Promoter

T7

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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Human_TFAM_HMGA_pET28

Plasmid

#167326

Purpose

Expresses TFAM HMGA mutant in bacterial cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Tags

6XHis

Expression

Bacterial

Mutation

Truncation mutant containing HMGA domain (43-122)

Promoter

T7

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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Human_TFAM_HMGA_linker_pET28

Plasmid

#167327

Purpose

Expresses TFAM HMGA+linker mutant in bacterial cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Tags

6XHis

Expression

Bacterial

Mutation

Truncation mutant containing HMGA and linker doma…

Promoter

T7

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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Human_TFAM_HMGB_ctail_pET28

Plasmid

#167330

Purpose

Expresses TFAM HMGB+C-tatil mutant in bacterial cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Tags

6XHis

Expression

Bacterial

Mutation

Truncation mutant containing HMGB and C-tail doma…

Promoter

T7

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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Human_TFAM_dC_pET28

Plasmid

#167328

Purpose

Expresses TFAM dC-tail mutant in bacterial cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Tags

6XHis

Expression

Bacterial

Mutation

Truncation mutant containing HMGA, linker and HMG…

Promoter

T7

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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SPIC_pet28a

Plasmid

#131654

Purpose

Bacterial expression of ETS gene SPIC

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

SPIC (SPIC Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pET28P_dCrk

Plasmid

#131145

Purpose

Expresses 6XHis::dCrk (based on crk isoform RA).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

Crk (Crk Fly)

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pET21d_SNORD78_probe

Plasmid

#73072

Purpose

antisense probe for detection of human SNORD78 (C/D box snoRNA U78)

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

SNORD78 (SNORD78 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only
pET29b-PD

Plasmid

#110168

Purpose

Expresses propanediol dehydratase in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Science. 2017 Feb 10;355(6325). pii: 355

Insert

Propanediol Dehydratase

Use

Tags

His6 and S-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pET22b_PfSSB_W166C

Plasmid

#103001

Purpose

The expressed protein is a Plasmodium falciparum single-stranded DNA-binding protein, PfSSB (C93A,W166C), used for obtaining a fluorescent adduct at the cysteine

Depositor

Martin Webb

Article

Chisty et al PLoS One. 2018 Feb 21;13(2):e0193272. do

Insert

PfSSB_W166C

Use

Tags

Expression

Bacterial

Mutation

C93A, W166C

Promoter

T7

Available since

May 24, 2018

Availability

Academic Institutions and Nonprofits only

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pET21a_Msm_RbpA

Plasmid

#87928

Purpose

Mycobacterium smegmatis RpbA in pET21a (ampR) used to co-express with Msm SigA for crystallization purposes

Depositor

Seth Darst

Article

Hubin et al Elife. 2017 Jan 9;6. pii: e22520. doi: 1

Insert

RbpA

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7P

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pET24a_UDP_Xase_E162

Plasmid

#73160

Purpose

Expresses in E. coli, S. pneumoniae TIGR4 UDP-Xase SP_1669 mutant E162A

Depositor

Sandra Gabelli

Article

Duong-Ly et al PLoS One. 2013 May 15;8(5):e64241. doi:

Insert

pET24a

Use

Tags

Expression

Bacterial

Mutation

change glutamate 162 to alanine

Promoter

Available since

March 22, 2016

Availability

Academic Institutions and Nonprofits only
pET24_BD3179

Plasmid

#73142

Purpose

Expresses in E.coli B. bacteriovorus Bd3179

Depositor

Sandra Gabelli

Article

de la Peña et al PLoS One. 2015 Nov 2;10(11):e0141716. do

Insert

Bd3179

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 22, 2016

Availability

Academic Institutions and Nonprofits only