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Plasmid#231986PurposeEmpty backbone for expression of Ovalbumin with Bi-cistronic expression ZsGreenDepositorInsertOVA-2A-ZsGreen
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHIV-EF1a-Il1a-ZsGreen
Plasmid#231987PurposeExpress IL-1alpha-ZsGreen fusion proteinDepositorInsertIL-1alpha-ZsGreen fusion construct
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE Myc-epitope donor ORF0
Plasmid#200374PurposeTagging endogenously expressed proteins with Myc epitope, C-terminalDepositorInsertMyc epitope
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE Myc-epitope donor ORF1
Plasmid#200375PurposeTagging endogenously expressed proteins with Myc epitope, C-terminalDepositorInsertMyc epitope
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE Myc-epitope donor ORF2
Plasmid#200376PurposeTagging endogenously expressed proteins with Myc epitope, C-terminalDepositorInsertMyc epitope
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHis6-SUMO-g0s2
Plasmid#179293PurposeE. coli expression plasmid (T7 promoter) for full-length human G0S2 with N-terminal His6 + SUMO + TEV protease cleavage siteDepositorInsertG0S2 (G0S2 Human)
UseTagsHis6 + SUMO + TEV protease cleavage siteExpressionBacterialMutationfull-length G0S2, residues 1-103 from accession n…PromoterT7Available sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE GS-gRNA (hSyn-Cre) backbone
Plasmid#200368PurposeBackbone plasmid for cloning HiUGE GS-gRNA (with hSyn-Cre)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationNAPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIV-EGFP.Flag-EZH2-mt2
Plasmid#220245PurposeExpresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.DepositorInsertEZH2 mt 2 (EZH2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationF32A, R34A, D36A, K39A, PRKKKR494-499NAAIRSPromoterEF-1αAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHIV-EGFP.Flag-EZH2-mt2*
Plasmid#220246PurposeExpresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.DepositorInsertEZH2 mt 2* (EZH2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPRKKKR494-499NAAIRSPromoterEF-1αAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE GS-gRNA (hSyn-Cre) mTubb3
Plasmid#200369PurposePositive control plasmid expressing mouse Tubb3 GS-gRNA (with hSyn-Cre)DepositorInsertMouse Tubb3 gRNA and Cre
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIS-MDH1-HIS
Plasmid#184558PurposeBacterial expression of human MDH1 with HIS TagDepositorInsertMalate dehydrogenase 1 (MDH1 Human)
UseTags6xHISExpressionBacterialMutationPromoterAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIV-ODD-EGFP-IRES-dTomato
Plasmid#87218Purposelentiviral expression of ODD-EGFPDepositorInsertHIF-1a ODD domain (HIF1A Human)
UseLentiviralTagsEGFPExpressionMammalianMutationODD domainPromoterEF1Available sinceMarch 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHIE323-LPIV Puro-P2A-miRFP720 ZF6x12-C-DsRed-Express2(x6) phEF1α-BlastR
Plasmid#201539PurposeLanding pad integration vector used to generate ZF6 synTF reporter line (HIE156), assembled with pPD1178 (destination vector), pHIE324-329 (TUPV1-6), pHIE280 (TUPV7), pPD1157 (linker)DepositorInsertsPuromycinR-P2A-miRFP720
ZF6x12-C-DsRed-Express2(x6)
BlasticidinR
UseTagsExpressionMammalianMutationPromoterNone, ZF6x12(C)_YB_TATA Minimal Promoter, and hEF…Available sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
L1PB26- pAHP6--PhiC31-NLS-tUBQ1 + pGATA23--Bxb1-SAUR-tUBQ10
Plasmid#219701PurposeLevel 1 PhiC31 and Bxb1 integrase construct for tracking of lateral root development: pAHP6 promoter for PhiC31 and pGATA23 promoter for Bxb1, NLS tag for PhiC31 and RNA degradation tag for Bxb1DepositorInsertpAHP6::PhiC31-NLS:tUBQ1 + pGATA23::Bxb1-SAUR:tUBQ10
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHIV-NAT-T2A-hCD52-eGFP-NFATC2IP-SPR
Plasmid#221447PurposeLentiviral mammalian expression vector of eGFP-tagged NFATC2IP ORF that is fused with S-phase restricted (SPR) degron at its C-terminusDepositorInsertNFATC2IP (NFATC2IP Human)
UseLentiviralTagseGFPExpressionMammalianMutationSPR degron: hGEM (1-110) fragment that is fused t…PromoterCMVAvailable sinceJuly 3, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHIV-NAT-FLAG-CIP2A FL (sg2R)
Plasmid#222624PurposeLentiviral vector that expresses Flag-tagged sgRNA resistant CIP2A in mammalian cellsDepositorInsertCIP2A (CIP2A Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterEF-1-alpha promoterAvailable sinceJuly 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSL006_phiC31_neo-5xTetO-pEF-H2B-mCitrine-pRSV-H2B-mCherry
Plasmid#179425PurposeDual fluorescent reporter construct with no spacer, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integrationDepositorInsertTRE-cit-(nospacer)-mch
UseTagsExpressionMammalianMutationPromoterpEF alpha, pRSVAvailable sinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
PhiC31-Neo-ins-5xTetO-pEF-H2B-Citrine-ins
Plasmid#78099PurposeNuclear fluorescent reporter in mammalian cells, phiC31 attB for integration, can be silenced with TetR-CR or rTetR-CR (e.g. CR can be KRAB or DNMT3B).DepositorInsertHistone H2B - Citrine (H2BC21 Synthetic, Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoter5xTetO pEF alphaAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHIV-NAT-FLAG-CIP2A ∆NES (del561-625)
Plasmid#222625PurposeLentiviral vector that expresses Flag-tagged CIP2A NES mutant in mammalian cellsDepositorInsertCIP2A (CIP2A Human)
UseLentiviralTagsFlagExpressionMammalianMutationDeletion of the residues 561-625 (ΔNES)PromoterEF-1-alpha promoterAvailable sinceJuly 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMH003_phiC31_neo-5xTetO-pEF-H2B-mCitrine-coreHS4-pRSV-H2B-mCherry
Plasmid#179431PurposeDual fluorescent reporter construct with single core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integrationDepositorInsertTRE-cit-coreHS4-mch (SC)
UseTagsExpressionMammalianMutationPromoterpEF alpha, pRSVAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only