Addgene

We narrowed to 465 results for: PD1

Showing: 181 - 200 of 465 results

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pAAV asyn WT

Plasmid

#36055

Depositor

Hilal Lashuel

Article

Oueslati et al J Neurosci. 2012 Feb 1;32(5):1536-44.

Insert

alpha-synuclein WT (SNCA Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pHR-SNAP-PD-1

Plasmid

#223572

Purpose

Lentiviral expression of human PD-1 with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

Insert

PD-1 (PDCD1 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Expression

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV asyn S87A

Plasmid

#36056

Depositor

Hilal Lashuel

Article

Oueslati et al J Neurosci. 2012 Feb 1;32(5):1536-44.

Insert

alpha-synuclein S87A (SNCA Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

S87A

Promoter

CMV

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only
pAAV asyn S87E

Plasmid

#36057

Depositor

Hilal Lashuel

Article

Oueslati et al J Neurosci. 2012 Feb 1;32(5):1536-44.

Insert

alpha-synuclein S87E (SNCA Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

S87E

Promoter

CMV

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only
t2. pT7-7-SNCA-5Lys

Plasmid

#107294

Purpose

Plasmid for the expression of the construct SNCA-5Lys. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Lys and 7 Gly

Depositor

Dmytro Yushchenko

Article

Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

Insert

SNCA-5Lys (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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r. pT7-7-SNCA-5Asn

Plasmid

#107295

Purpose

Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Asn and 7 Gly

Depositor

Dmytro Yushchenko

Article

Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

Insert

SNCA-5Asn (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
s2. pT7-7-SNCA-5Asp

Plasmid

#107293

Purpose

Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Asp and 7 Gly

Depositor

Dmytro Yushchenko

Article

Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

Insert

SNCA-5Asp (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-PD-1-SNAP

Plasmid

#223576

Purpose

Lentiviral expression of human PD-1 with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

Insert

PD-1 (PDCD1 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Expression

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
VN-alpha Synuclein

Plasmid

#89470

Purpose

expresses human alpha Synuclein fused to the N-terminal part of Venus

Depositor

Bradley Hyman, Tiago Outeiro

Article

Outeiro et al PLoS One. 2008 Apr 2;3(4):e1867. doi: 10

Insert

SNCA (SNCA Human)

Use

Tags

N-terminal part of Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-7 asyn A53T

Plasmid

#105727

Purpose

Bacterial expression of mutant human alpha synuclein

Depositor

Hilal Lashuel

Article

Rospigliosi et al J Mol Biol. 2009 May 22;388(5):1022-32.

Insert

alpha-synuclein A53T (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

A53T

Promoter

T7

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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alpha Synuclein-VC

Plasmid

#89471

Purpose

expresses human alpha Synuclein fused to the C-terminal part of Venus

Depositor

Bradley Hyman, Tiago Outeiro

Article

Outeiro et al PLoS One. 2008 Apr 2;3(4):e1867. doi: 10

Insert

SNCA (SNCA Human)

Use

Tags

C-terminal part of Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-7 asyn A30P

Plasmid

#105726

Purpose

Bacterial expression of mutant human alpha synuclein

Depositor

Hilal Lashuel

Article

Rospigliosi et al J Mol Biol. 2009 May 22;388(5):1022-32.

Insert

alpha-synuclein A30P (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

A30P

Promoter

T7

Available since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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1a. pETM11-SUMO-SNCA-GFP

Plasmid

#107292

Purpose

Plasmid for the expression of the construct alpha-Synuclein-GFP, which contains N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility

Depositor

Dmytro Yushchenko

Article

Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

Insert

SNCA-GFP (SNCA Human)

Use

Tags

His-tag and SUMO-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
r-22. pT7-7-SNCA-9Asn

Plasmid

#107297

Purpose

Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 22 aa C-terminal moiety, which consists of 9 Asn and 13 Gly

Depositor

Dmytro Yushchenko

Article

Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

Insert

SNCA-9Asn (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 7, 2018

Availability

Academic Institutions and Nonprofits only

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r-6. pT7-7-SNCA-2Asn

Plasmid

#107296

Purpose

Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 6 aa C-terminal moiety, which consists of 2 Asn and 4 Gly

Depositor

Dmytro Yushchenko

Article

Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

Insert

SNCA-2Asn (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-SNAP-PD-1 I126A

Plasmid

#223573

Purpose

Lentiviral expression of human PD-1 (I126A) with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

Insert

PD-1 (PDCD1 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Expression

Mutation

I126A

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pPPI4-Strep-SNAP-PD-1

Plasmid

#223569

Purpose

Protein purification of human PD-1 with Twin-Strep and SNAP tags from mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

Insert

PD-1 (PDCD1 Human)

Use

Tags

Twin-Strep, SNAP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-7 asyn WT

Plasmid

#36046

Depositor

Hilal Lashuel

Article

Paleologou et al J Biol Chem. 2008 Jun 13;283(24):16895-9

Insert

alpha-synuclein (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
PD-1-miSFIT-6-C, 16-C

Plasmid

#124681

Purpose

Tuning expression of PD-1

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

PD-1 (PDCD1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

minimal CMV

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-7 aSyn C141

Plasmid

#108866

Purpose

Expresses human WT alpha synuclein with a C-terminal cysteine

Depositor

Gabriele Kaminski Schierle

Article

Stephens et al bioRxiv 2022.10.04.510646

Insert

alpha synuclein (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

C141 mutation to allow C-terminal maleimide dye l…

Promoter

T7

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge