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pCMV-GFP-CENPBpA-fixed-length-clone-4

Plasmid

#120182

Purpose

Randomly picked clone 4 of the pCMV-GFP-CENPBpA-fixed-length library

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB poly(A) site

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-CENPBpA-fixed-length-clone-3

Plasmid

#120181

Purpose

Randomly picked clone 3 of the pCMV-GFP-CENPBpA-fixed-length library

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB poly(A) site

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a human Tankyrase2 full length Y920E

Plasmid

#132639

Purpose

expresses human Tankyrase2 full length Y920E in E. coli

Depositor

Wenqing Xu

Article

DaRosa et al Protein Sci. 2016 Sep;25(9):1744-52. doi

Insert

Tankyrase2 Y920E

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 human PP2A B56α full length

Plasmid

#132635

Purpose

expresses human PP2A B56α full length in E. coli

Depositor

Wenqing Xu

Article

Sangodkar et al J Clin Invest. 2017 Jun 1;127(6):2081-20

Insert

PP2A B56α

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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Myc-tagged human full length TCF4E pcDNA3

Plasmid

#32738

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

TCF4E (TCF7L2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti-Zyxin-Full Length (aa1-564)-EGFP

Plasmid

#187692

Purpose

Visualization of Zyxin

Depositor

Mary Beckerle

Article

Hoffman et al Mol Biol Cell. 2012 May;23(10):1846-59.

Insert

Zyxin (Zyx Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac1 human full-length CENP-E-mNeon-6xHis

Plasmid

#180484

Purpose

pFastBac1 human CENP-E-mNeon-His, containing a 3c protease cleavage site between mNeon and His tag. Codon optimized for expression in Sf9 cells

Depositor

Julie Welburn

Article

Craske et al Open Biol. 2022 Mar;12(3):210389. doi: 1

Insert

CENP-E (CENPE Human, human, Synthetic)

Use

Tags

mNeon-6xHis

Expression

Insect

Mutation

Promoter

polyhedrin promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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Tet-off Full length Tau (0N4R, P301L/V337M)

Plasmid

#188573

Purpose

Dox-repressible expression of the 0N4R Tau isoform

Depositor

Franz-Ulrich Hartl

Article

Saha et al Nat Commun. 2023 Feb 2;14(1):560. doi: 1

Insert

0N4R Tau (MAPT Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

P301L and V337M

Promoter

pTight TRE

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-(mcs) human CSF1 full length-IRES-Puro

Plasmid

#86797

Purpose

retroviral expression of CSF-1

Depositor

Martine Roussel, Charles Sherr

Article

Roussel et al Nature. 1987 Feb 5-11;325(6104):549-52.

Insert

CSF-1 (CSF1 Human)

Use

Retroviral

Tags

IRES-Puro

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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M57 pPET28a-TEV-full-length human beta catenin

Plasmid

#17198

Depositor

Randall Moon

Insert

beta catenin (CTNNB1 Human)

Use

Tags

HIS

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only

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M58 pPET28b-TEV-full-length zebrafish beta catenin

Plasmid

#17199

Depositor

Randall Moon

Insert

beta catenin (ctnnb1 Zebrafish)

Use

Tags

HIS

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
WNV isolate WN NY 2000-crow3356 NS5 RdRp full length

Plasmid

#228662

Purpose

Bacterial expression of the West Nile virus NS5 RdRp protein for biochemical assay

Depositor

ASAP Discovery, Frank von Delft

Insert

West nile NS5 RdRp

Use

Tags

His-SUMO

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pFastBac dual_Human full length CDK7(W132R, S164D, T170E)

Plasmid

#217013

Purpose

Expression of CDK7 in insect cells

Depositor

Manjeet Mukherjee

Article

Mukherjee et al Structure. 2024 Jun 9:S0969-2126(24)0018

Insert

CDK7(W132R, S164D, T170E) (CDK7 Human)

Use

Tags

TEV cleavable his tag

Expression

Insect

Mutation

CDK7(W132R, S164D, T170E)

Promoter

Available since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroX-Tight-Pur-Trim69 Full length, isoform A (codon optimized)

Plasmid

#199566

Purpose

Stable and dox. inducible expression of Trim69 Full length, isoform A (codon optimized) after retroviral-mediated gene transfer

Depositor

Andrea Cimarelli, Yuxin Song

Article

Song et al Proc Natl Acad Sci U S A. 2022 Oct 25;11

Insert

Trim69 Full length, isoform A (codon optimized) (TRIM69 Human)

Use

Retroviral; Allows for puromycin selection

Tags

Flag

Expression

Mutation

codon optimized

Promoter

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac dual_Human full length _CDK7 (S164D, T170E)_del327-346

Plasmid

#217012

Purpose

Expression of CDK7 in insect cells

Depositor

Manjeet Mukherjee

Article

Mukherjee et al Structure. 2024 Jun 9:S0969-2126(24)0018

Insert

CDK7 (S164D, T170E)_del327-346 (CDK7 Human)

Use

Tags

TEV cleavable his tag

Expression

Insect

Mutation

(S164D, T170E)_del327-346

Promoter

Available since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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Human messenger-RBP sgRNA library

Pooled Library

#168791

Purpose

CRISPR library targets sub-pool of human mRNA binding proteins

Depositor

Martin Turner

Article

Turner et al CRISPR J. 2021 Jun;4(3):427-437. doi: 10

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

July 30, 2021

Availability

Academic Institutions and Nonprofits only
Mouse messenger-RBP sgRNA library

Pooled Library

#169082

Purpose

CRISPR library targeting mouse genes

Depositor

Martin Turner

Article

Turner et al eLife. 2022 Apr 22;11. pii: 72313. doi:

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only
pCORE-UH

Plasmid

#72239

Purpose

Contains the CORE cassette with KlURA3 and hyg markers

Depositor

Michael Resnick, Francesca Storici

Article

Storici et al Genet Eng (N Y). 2003;25:189-207.

Inserts

KlURA3
hyg

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pCORE-UK

Plasmid

#72238

Purpose

Contains the CORE cassette with convergent KlURA3 and KanMX4 markers

Depositor

Michael Resnick, Francesca Storici

Article

Storici et al Genet Eng (N Y). 2003;25:189-207.

Inserts

KlURA3
kanMX4

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pZS2-123

Plasmid

#26598

Depositor

Michael Elowitz

Article

Cox et al J Biol Eng. 2010 . 4():10.

Insert

Synthetic

Use

Tags

Expression

Bacterial

Mutation

N/A

Promoter

Available since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only

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