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Plasmid#158641PurposeLentiviral expression vector for constitutive expression of V5-tagged OAS1.DepositorInsertOAS1 (OAS1 Human)
UseLentiviralTagsV5ExpressionMutationPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
TagBFP_A1aY1
Plasmid#158752PurposeLentiviral vector for expression of TagBFP_A1aY1 in mammalian cells for detection of tyrosinated microtubules.DepositorInsertTagBFP-T_A1aY1
UseLentiviralTagsTagBFPExpressionMammalianMutationPromoterCAG (CMV enhancer and chicken beta actin promoter)Available sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-TET3G-T2A-Puro
Plasmid#158067PurposeEncodes the Tet3G transactivator and puromycin resistance.DepositorInsertpCAG-TET3G-T2A-Puro
UseAAVTagspuromycin reistance geneExpressionMammalianMutationPromoterpCAGAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-TMPRSS2-blast
Plasmid#158458PurposeConstitutive lentiviral expression of human TMPRSS2.DepositorInsertTMPRSS2 (TMPRSS2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
scFLARE1-p2A-eGFP
Plasmid#158699PurposeMammalian expression of scFLARE1 component bearing a p2A-eGFP as transfection markerDepositorInsertscFLARE1-p2A-eGFP
UseAAVTagsFlag, V5. eGFPExpressionMammalianMutationTruncated form of TEV protease (220-242 amino aci…PromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDOC-K-glmS-GFPfor
Plasmid#158059PurposeDonor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the forward orientation relative to the kanamycin resistance selectable markerDepositorInsertGreen Fluorescent Protein optimised for excitation with UV light
UseSynthetic BiologyTagsExpressionBacterialMutationPromoteracpPAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pW34
Plasmid#158210PurposeLuxCDABE operon (R6K ori) under T7, in loxP and lox5171 sites.DepositorInsertLuxABCDE
UseTagsExpressionMutationPromoterAvailable sinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPP441
Plasmid#158801PurposePlasmid containing Homo sapiens codon optimized CasΦ-2 and a SapI-GG stuffer spacer for editing in human cells.DepositorInsertCasPhi-2
UseTags2xSV40NLS-2xFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-TMPRSS2-puro
Plasmid#158457PurposeConstitutive lentiviral expression of human TMPRSS2.DepositorInsertTMPRSS2 (TMPRSS2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRDA_256
Plasmid#158581PurposeLentiviral expression of BE3.9max and customizable gRNADepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationCas9 D10APromoterAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-dSpRY-Act3.0
Plasmid#158416PurposeCRISPR-Act3.0 system containing dSpRYCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.DepositorInsertdSpRY-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
GAL FLAG25QDProGFP p306
Plasmid#15831DepositorInserthtt 25Q Delta Pro (HTT Human)
UseTagsFLAG and GFPExpressionYeastMutationProline-rich region removedPromoterAvailable sinceOct. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131-tRNA2.0
Plasmid#158393PurposeGolden Gate entry vector to express the 1st gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 SARS-CoV-2 E
Plasmid#158080PurposeExpresses SARS-CoV-2 envelope protein in mammalian cellsDepositorInsertSARS-CoV-2 envelope (E )
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
6xHis-TagRFP-T_A1aY1
Plasmid#158754PurposeExpression of 6x His tagged TagRFP-T_A1aY1 in mammalian cells for detection of tyrosinated microtubules.DepositorInsert6xHistidine_TagRFP-T_A1aY1
UseTags6x-Histidine tag and TagRFP-TExpressionMammalianMutationPromoterCMV PromoterAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBJB20 - eft-3p::mitofilin::Mac::GFP::unc-54 3'UTR
Plasmid#158967PurposeUbiquitous expression of mtON in C. elegansDepositorInserteft-3p::mitofilin::Mac::GFP::unc-54 3'UTR
UseTagsGFPExpressionWormMutationPromotereft-3Available sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT_025
Plasmid#158579PurposeLentiviral expression of a customizable open reading frameDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
mTAGBFP2 Peroxisome visual barcode
Plasmid#158668Purposetagging clonal populations with Peroxisomal mTAGBFP2 fluorescent protein. Adding Peroxisome localization signal to mTAGBFP2 WCDepositorInsertmTAGBFP2
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR E3 pR8A L3-L2
Plasmid#158735PurposePlant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last NN)DepositorInsertTALEN ORF without TAL repeat
UseTALENTagsExpressionMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR E3 pR5A L3-L2
Plasmid#158732PurposePlant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last HD)DepositorInsertTALEN ORF without TAL repeat
UseTALENTagsExpressionMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only