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Plasmid#182209PurposeBacterial expression of frSkylan-SDepositorInsertfrSkylan-S
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMay 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Sema3a(S)-Fc-His
Plasmid#72138PurposeExpresses the Sema3A protein (truncated at cleavage site P1; ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertSema3a (Sema3a Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
Beclin-HA S-D
Plasmid#46995PurposeExpresses Beclin1 in mammalian cellsDepositorInsertbecn1
UseTagsHAExpressionMammalianMutationS14DPromoterAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET32a-Spike-S-4P
Plasmid#165094Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 spike protein site 550-570, 785- 805, 810-830 and 1146 1166 (S SARS-CoV2)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 2, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0M-S-mClover3-EC18157
Plasmid#137076PurposeGolden Gate Level 0 S module for N-terminal fluorescent protein taggingDepositorInsertmClover3 DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS/D614G
Plasmid#164566PurposeSARS-CoV-2 Spike protein with D614G mutation (S-GSAS-D614G variant)DepositorInsertSpike (S-GSAS-D614G variant)
UseTags2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…ExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sema3f(S, +)-AP-His
Plasmid#72023PurposeExpresses the Sema3F protein (truncated at cleavage site P1; ie, short and contains no deletion in exon 3), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSema3f (Sema3f Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
Plxnb2(S)-Fc-His
Plasmid#72129PurposeExpresses the N-terminal extracellular region of the PlexinB2 protein following proteolytic cleavage (ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertPlxnb2 (Plxnb2 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-HRV3C/D614G
Plasmid#164568PurposeSARS-CoV-2 Spike protein with furin site mutated to HRV3C site and D614G mutation (S-HRV3C-D614G variant)DepositorInsertSpike (S-HRV3C-D614G variant)
UseTags2X Strep-Tag II and 8X His tagExpressionMammalianMutationD614GPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21b - S. thermophilus DltB
Plasmid#132607Purposeexpresses S. thermophilus DltB in E. coliDepositorInsertDltb
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
Sema3c(S)-Fc-His
Plasmid#72142PurposeExpresses the Sema3C protein (truncated at cleavage site P1; ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertSema3c (Sema3c Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema3c(S)-AP-His
Plasmid#72016PurposeExpresses the Sema3C protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSema3c (Sema3c Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema3a(S)-AP-His
Plasmid#72012PurposeExpresses the Sema3A protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSema3a (Sema3a Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-S-501V2-FLAG
Plasmid#177096Purpose501V2 variant S-protein with c-terminal FLAG tagDepositorInsertS-protein 501V2 (S SARS-CoV-2)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAGGS-S-P1-FLAG
Plasmid#177095PurposeP1 variant S-protein with c-terminal FLAG tagDepositorInsertS-protein P1 (S SARS-CoV-2)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
YFP-synDAT-S/D
Plasmid#48793PurposeExpresses YFP-tagged human DAT with first 5 N-terminal serines mutated to aspartates in pCIHygroDepositorInsertYFP-synhDAT-S/D (SLC6A3 Human)
UseTagsYFPExpressionMammalianMutationSer2, Ser4, Ser7, Ser12, and Ser13 mutated simult…PromoterAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPIC-mouseKir3.2-mC-S
Plasmid#140745PurposeExpression of mouse Kir3.2 fused to mCherry and Streptag IIDepositorInsertKir3.2 (Kcnj6 Mouse)
UseTagsmCherry with Streptag IIExpressionYeastMutationPromoterAOX1Available sinceMay 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCTD26-S>A10-17
Plasmid#112826PurposepSMF2 with all S to A mutations in only repeats 10-17.DepositorInsertRPB1 (RPO21 Budding Yeast)
UseTagsExpressionYeastMutationpSMF2 with all S to A mutations in only repeats 1…PromoterNative promoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCTD26-S>A2-9
Plasmid#112825PurposepSMF2 with all S to A mutations in only repeats 2-9.DepositorInsertRPB1 (RPO21 Budding Yeast)
UseTagsExpressionYeastMutationpSMF2 with all S to A mutations in only repeats 2…PromoterNative promoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCTD26-S>A18-25
Plasmid#112827PurposepSMF2 with all S to A mutations in only repeats 18-25.DepositorInsertRPB1 (RPO21 Budding Yeast)
UseTagsExpressionYeastMutationpSMF2 with all S to A mutations in only repeats 1…PromoterNative promoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only