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Showing: 451 - 500 of 1188 results

  4. W45A mutant of H3K9me3 biosensor

    Plasmid
    #120808
    Purpose
    FRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capability
    Depositor
    Yingxiao Wang
    Article
    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p
    Insert
    Truncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Tryptophan 45 is mutated to Alanine 45 on HP1 dom…
    Promoter
    CMV
    Available since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    120808-plasmid-map-sequence-id-235136

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  12. H2B-LSSmGFP

    Plasmid
    #186539
    Purpose
    Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to histone 2B
    Depositor
    Gregory Petsko
    Article
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Insert
    LSSmGFP
    Use
    Tags
    Human Histone 2B (D26G and V119I mutations)
    Expression
    Mammalian
    Mutation
    hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K
    Promoter
    CMV
    Available since
    Nov. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    186539-plasmid-map-sequence-id-368219

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  36. GFP-X3

    Plasmid
    #115568
    Purpose
    Synthetic acetylation substrate consisting of GFP fusion with 3 repeats of the Gcn5 consensus motif
    Depositor
    Michael Downey
    Article
    Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62
    Insert
    Green Fluorescent Protein
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Three repeats of the Gcn5 consensus motif are tag…
    Promoter
    ADH1
    Available since
    Oct. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    115568-plasmid-map-sequence-id-226342

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  44. Spt2-K166R-GFP

    Plasmid
    #115573
    Purpose
    Expresses yeast Spt2-GFP fusion protein mutated from lysine to arginine at site 166
    Depositor
    Michael Downey
    Article
    Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62
    Insert
    SPT2
    Use
    Tags
    GFP
    Expression
    Bacterial and Yeast
    Mutation
    Lysine 166 mutated to Arginine
    Promoter
    Available since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only

  45. GFP-X2

    Plasmid
    #115567
    Purpose
    GFP tethered to two repeats of the Gcn5 consensus motif
    Depositor
    Michael Downey
    Article
    Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62
    Insert
    Green Fluorescent Protein
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Two repeats of the Gcn5 consensus motif are tagge…
    Promoter
    ADH1
    Available since
    Oct. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    115567-plasmid-map-sequence-id-226345

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  46. GFP-X1

    Plasmid
    #115566
    Purpose
    GFP tethered to a single repeat of the Gcn5 consensus motif
    Depositor
    Michael Downey
    Article
    Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62
    Insert
    Green Fluorescent Protein
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Single Gcn5 consensus motif repeat tethered to C-…
    Promoter
    ADH1
    Available since
    Oct. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    115566-plasmid-map-sequence-id-226330

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Showing: 451 - 500 of 1188 results