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Plasmid#160804PurposeExpress C-terminal ISC mutant POLE1DepositorInsertPOLE1 C-ISCmut (POLE Human)
UseRetroviralTags3xFLAGExpressionMutationshPOLE_1 and shPOLE2 resistant, C2221S, C2224S, C…PromoterAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MARK2-K82H-CR
Plasmid#229425PurposeLentiviral or overpexression of cDNADepositorInsertMARK2-K82H (MARK2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationKinase-dead mutant K82H; silent mutation CRISPR r…PromoterEFSAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MARK3-GK-CR
Plasmid#229424PurposeLentiviral or overpexression of cDNADepositorInsertMARK3-GK-CR (MARK3 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationGatekeeper mutation M132G; silent mutation CRISPR…PromoterEFSAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MARK2-GK-CR
Plasmid#229423PurposeLentiviral or overpexression of cDNADepositorInsertMARK2-GK-CR (MARK2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationGatekeeper mutation M129G; silent mutation CRISPR…PromoterEFSAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast ACSL3
Plasmid#173165PurposeRetroviral vector to express sgRNA resistant human ACSL3DepositorInsertAcyl-CoA Synthetase Long Chain Family Member 3 (ACSL3 Human)
UseRetroviralTagsExpressionMutationSynonymous mutations at sgRNA sitesPromoterMMLVAvailable sinceAug. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUt-mNF-Cas13d
Plasmid#224789PurposeLPUtopia matching RMCE donor plasmid with mCherry-P2A-RfxCas13d Negative-Feedback circuit. Use BlastR for positive selection and HSV-TK for negative selection.DepositorInsertmCherry-P2A-RfxCas13d
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMV-d2iAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUNO mouse CD274 + 3'UTR WT full length
Plasmid#107012PurposeExpresses murine PD-L1 with the 3'UTRDepositorInsertCD274 (Cd274 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLentiCMVblast_SLC38A2_wt
Plasmid#156180PurposeCMV-driven expression of sgRNA-resistant SLC38A2 wild-type cDNA.DepositorInsertSLC38A2 (SLC38A2 Human)
UseTagsExpressionMammalianMutationSilent mutations to prevent targeting by sgRNA se…PromoterCMVAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLentiCMVblast_SLC38A2_N82A
Plasmid#156181PurposeCMV-driven expression of sgRNA-resistant SLC38A2 N82A mutant cDNA.DepositorInsertSLC38A2 (SLC38A2 Human)
UseTagsExpressionMammalianMutationSilent mutations to prevent targeting by sgRNA: T…PromoterCMVAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast HA-GPAT4-ΔNs5
Plasmid#173169PurposeRetroviral vector to express sgRNA resistant HA tagged human GPAT4 with mutation in CHP1 binding siteDepositorInsertGlycerol-3-Phosphate Acyltransferase 4 (GPAT4 Human)
UseRetroviralTagsHAExpressionMutationSynonymous mutations at sgRNA sites, amino acids …PromoterMMLVAvailable sinceAug. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast 3XFLAG-CHP1-myr_mut
Plasmid#173167PurposeRetroviral vector to express sgRNA resistant 3XFLAG tagged human CHP1 with myristoylation site mutationDepositorInsertCalcineurin Like EF-Hand Protein 1 (CHP1 Human)
UseRetroviralTags3XFLAGExpressionMutationSynonymous mutations at sgRNA sites, G2A, S6APromoterMMLVAvailable sinceAug. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
DinoIII-bsr
Plasmid#155027PurposeDinoflagelate backbone for blasticidin S-resistance gene (bsr)DepositorInsertBlasticidin-S deaminase
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-Cas9Bsd-W
Plasmid#68343PurposeLentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminusDepositorInsertEF1a-Cas9 cassette, WPRE
UseCRISPR and LentiviralTagsCas9 fused with Flag-tag and a NLS at the N termi…ExpressionMutationPromoterEF1aAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-BsdCas9-W
Plasmid#67978PurposeLentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the N-terminusDepositorInsertEF1a-Cas9 cassette, WPRE
UseCRISPR and LentiviralTagsCas9 fused with Flag-tag and a NLS at the N termi…ExpressionMutationPromoterEF1aAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only