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Showing: 361 - 400 of 1441 results

  1. EW492 CMV-TO-UTRN-14ZF(N61K,H88F, I91E, K120F)-realdeImmLink-NZF(FLP-IN)

    Plasmid
    #236150
    Purpose
    Express mutated UTRN-14 zinc finger array (N62K, H89F, I91E, K121F) attached by a deimmunized linker to the NZF transcriptional activation under control of CMV promoter with two TetR binding sites
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Insert
    UTRN-14ZF-deImmLink-NZF
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N61K, H88F, I91E, K120F in UTRN-14ZF
    Promoter
    CMV promoter with two TetR binding sites
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236150-plasmid-map-sequence-id-468429

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  2. 5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9 probasin_mTQ2_FlpO

    Plasmid
    #68357
    Purpose
    The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and 8, and SMAD4 ex2 and ex 9 are expressed by the U6 promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Inserts
    5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9
    FlpO recombinase
    Use
    CRISPR
    Tags
    mTurquoise2 (BFP)
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and synthetic Probasin ARRx2
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68357-plasmid-map-sequence-id-122833

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  3. EW482 CMV-TO-ADAR2dd(E488Q, T501A)-PUF-9R-MARIAcomp(V241D, H243M) [D241C, M243Q] (FLP-IN)

    Plasmid
    #236128
    Purpose
    Plasmid encoding ADAR2dd attached to PUF-9R with deimmunizing and function-restoring mutations under control of CMV promoter with two TetR binding sites
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Insert
    ADAR2dd-PUF-9R (ADARB1 Synthetic, Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E488Q, T501A in ADAR2dd; V241C, H243Q in PUF-9R
    Promoter
    CMV promoter with two TetR binding sites
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236128-plasmid-map-sequence-id-468464

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  4. EW489 CMV-TO-ZNF35(5-8)-ZNF250(1-4)-(GGS)x8[G2V G4W S6L G7L G10W G11D G13L G17M G19I G22M G23Q S24Q]-FKBP (FLP-IN)

    Plasmid
    #236147
    Purpose
    Plasmid encoding the ZNF35/ZNF250 zinc finger array attached by a deimmunized linker to FKBP1A, under control of CMV promoter with two TetR binding sites
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Insert
    ZNF35(5-8)-ZNF250(1-4)-deImmunLink-FKBP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter with two TetR binding sites
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236147-plasmid-map-sequence-id-468434

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  5. pDGB3_alpha1 nptII-DISTC02-CBS-FRT-Rep-RepA-tNos-DISTC19-CBS-miniDFR-FLP-tNos-PROXC02-pNos-CUP2-Gal4AD-tNos-SF (GB4697)

    Plasmid
    #225440
    Purpose
    Module for copper and flippase-regulated expression of BeYDV Rep/RepA, including the TU for copper-regulated FLP, the TU for constitutive expression of CUP2-GAL4, and the nptII cassette.
    Depositor
    Diego Orzaez
    Insert
    nptII-DISTC02-CBS-FRT:Rep/RepA:tNos-DISTC19-CBS:miniDFR:FLP:tNos-PROXC02-pNos:CUP2:Gal4AD:tNos-SF
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225440-plasmid-map-sequence-id-447732

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  9. pAAV-hSyn-fDIO-mCherry-WPREpA

    Plasmid
    #177328
    Purpose
    Control vector for Flp-dependent DREADD constructs
    Depositor
    Ulrik Gether
    Insert
    mCherry
    Use
    AAV; Flp-frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human Synapsin-1
    Available since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177328-plasmid-map-sequence-id-349583

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  31. pNM3746

    Plasmid
    #154109
    Purpose
    loxP FLP FRT FRT3 landing site in a Golden Gate SapI GCG ACG assembly vector
    Depositor
    Michael Nonet
    Article
    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.
    Inserts
    LoxP-SEC-LoxP
    FRT::rpl-28p::GFP::His-58::FRT3
    mex-5p::FLP D5::sl2::mNG::glh2 3'
    Use
    Cre/Lox; Rmce
    Tags
    Expression
    Worm
    Mutation
    Promoter
    not applicable
    Available since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    154109-plasmid-map-sequence-id-306068

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  32. pAAV-hSyn-fDIO-HA-KORD-IRES-mCitrine-WPREpA

    Plasmid
    #154871
    Purpose
    Human synapsin-1 promoter; Flp-dependent KORD-IRES-mCitrine
    Depositor
    Ulrik Gether
    Insert
    KORD-IRES-mCitrine
    Use
    AAV; Flp-frt
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Human Synapsin-1
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    154871-plasmid-map-sequence-id-308834

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  33. pAAV-hSyn-fDIO-rM3D(Gs)-mCherry-WPREpA

    Plasmid
    #154869
    Purpose
    Human synapsin-1 promoter; Flp-dependent rM3D(Gs) DREADD-mCherry
    Depositor
    Ulrik Gether
    Insert
    rM3D(Gs)
    Use
    AAV; Flp-frt
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Human Synapsin-1
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    154869-plasmid-map-sequence-id-308828

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  34. pAAV-Ef1a-fDIO-JRGECO1a

    Plasmid
    #128317
    Purpose
    Expresses JRGECO1a under control of eukaryotic Ef1a promoter after Flp-dependent recombination.
    Depositor
    Patricia Jensen
    Has Service
    AAV1
    Insert
    NES-JRGECO1a
    Use
    AAV
    Tags
    His Tag
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1a
    Available since
    Sept. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    128317-plasmid-map-sequence-id-257202

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  35. pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA

    Plasmid
    #154868
    Purpose
    Human synapsin-1 promoter; Flp-dependent hM3D(Gq) DREADD-mCherry
    Depositor
    Ulrik Gether
    Has Service
    AAV Retrograde and AAV8
    Insert
    hM3D(Gq)-mCherry
    Use
    AAV; Flp-frt
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Human Synapsin-1
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    154868-plasmid-map-sequence-id-308827

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Showing: 361 - 400 of 1441 results