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Plasmid#49386PurposeExpresses APEX2 in the cytosol of mammalian cellsDepositorInsertFlag - APEX2 - NES
UseTagsFlagExpressionMammalianMutationK14D, W41F, E112K, A134P on soybean APX; improved…PromoterCMVAvailable sinceNov. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Connexin43-GFP-APEX2
Plasmid#49385PurposeExpresses a tandem GFP-APEX2 fusion to the C-terminus of connexin43 in mammalian cellsDepositorInsertConnexin43 - EGFP - APEX2
UseTagsExpressionMammalianMutationK14D, W41F, E112K, A134P on soybean APX; improved…PromoterCMVAvailable sinceNov. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
28A_APEX2_MSP1D1
Plasmid#170923PurposeExpress APEX2_MSP1D1 in Bl21DepositorInsertAPEX2-MSP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV_Ef1a_FLEX_mAPEX2_P2A_mRuby2
Plasmid#171938PurposeExpresses membrane targeted (palmitoylation) APEX2 and mRuby2 as separate proteinsDepositorInsertApex2, mRuby2
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEf1aAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
PM-APEX2
Plasmid#215557PurposeStable, inducible expression in plasma membraneDepositorInsertLyn11-EGFP-Flag tag-APEX2-NES
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
APEX2-ChBP
Plasmid#108878PurposeExpression of APEX2 fused to a nanobody against mCherryDepositorInsertAPEX2-ChBP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
APEX2-NLS
Plasmid#124617Purposeproximity biotinylationDepositorInsertAPEX2-NLS
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
LAMP1-APEX2
Plasmid#215555PurposeStable, inducible expression in lysosomeDepositorInsertLAMP1-EGFP-Flag tag-APEX2-NES
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRC-APEX2
Plasmid#72558PurposeExpression of cytosolic APEX2 in bacteriaDepositorInsertAPEX2
UseTagsHis6ExpressionBacterialMutationA134P relative to APEXPromoterAvailable sinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
mito-V5-APEX2
Plasmid#72480PurposeExpresses APEX2 in mitochondriaDepositorInsertAPEX2
UseTagsV5ExpressionMammalianMutationPromoterCMV promoterAvailable sinceApril 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
APEX2-OMM
Plasmid#79056PurposeSoybean APEX2 anchored to cytosolic face of outer mitochondrial membrane with C-terminal targeting sequence of last 31aa of human mitochondrial antiviral-signaling proteinDepositorInsertSoybean APEX2
UseLentiviralTagsflag and last 31 amino acids of human MAVSExpressionBacterial and MammalianMutationPromoterCMV immearly promotorAvailable sinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJJiaDEST-APEX2
Plasmid#149517PurposeN-termial APEX2 tag. Gateway destination vector for mammanlian expressionDepositorTypeEmpty backboneUseTagsFLAG-APEX2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
ERM-APEX2
Plasmid#79055PurposeSoybean APEX2 anchored to cytosolic face of ER membrane with N-terminal targeting sequence of residues 1-27 of ER-resident P450 oxidase 2C1.DepositorInsertAPEX2 anchored to the cytosolic face of the ER membrane
UseLentiviralTagsResidues 1-27 of P450 oxidase 2C1 and V5ExpressionBacterial and MammalianMutationPromoterCMV immearly promotorAvailable sinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
APEX2-GBP
Plasmid#67651PurposeMammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody).DepositorInsertAPEX2-GBP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
IMS-APEX2
Plasmid#79058PurposeExpresses APEX in the mitochondrial intermembrane space.DepositorInsertAPEX
UseTagsV5ExpressionBacterial and MammalianMutationPromoterT7 promoterAvailable sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET24b-APEX2
Plasmid#111702PurposeFor IPTG-inducible expression of APEX2 in E. coliDepositorInsertAPEX2
UseTags6xHis and V5ExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cyto-V5-APEX2
Plasmid#170572PurposeExpresses APEX2 in cytosolDepositorInsertAPEX2
UseLentiviralTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
APEX2-csGBP
Plasmid#108874PurposeExpression of APEX2 fused to a conditionally stable nanobody against GFPDepositorInsertAPEX2-csGBP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
PLIN2-V5-APEX2
Plasmid#170573PurposeA C-terminal APEX2 fusion to the mouse perilipin-2 protein PLIN2DepositorInsertPLIN2 (Plin2 Mouse)
UseLentiviralTagsV5-APEX2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_Sec61B-V5-APEX2
Plasmid#83411PurposeExpressed C-term tagged APEX2 on Sec61B in mammalian cellDepositorInsertSec61B-V5-APEX2 (SEC61B Synthetic, Human)
UseTagsAPEX2 and V5ExpressionMammalianMutationPromoterCMV-FAvailable sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only