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Showing: 1 - 20 of 315 results

  1. pMT3 p38

    Plasmid
    #12658
    Depositor
    John Kyriakis
    Insert
    p38alpha (MAPK14 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 21, 2006
    Availability
    Academic Institutions and Nonprofits only
    12658-plasmid-map-sequence-id-169265

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  2. P38-bio

    Plasmid
    #68518
    Purpose
    Expresses full-length P. vivax P38 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence
    Depositor
    Julian Rayner, Gavin Wright
    Article
    Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0
    Insert
    P38
    Use
    Tags
    rat CD4 d3+4, enzymatic biotinylation sequence
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Sept. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    68518-plasmid-map-sequence-id-120630

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  3. P38-bio

    Plasmid
    #47727
    Purpose
    Expresses enzymatically monobiotinylated full-length P38 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised P38
    Use
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47727-plasmid-map-sequence-id-68460

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  5. pPP381

    Plasmid
    #158798
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-2(D394A) in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Article
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    dCasPhi-2
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    RuvC-inactivating mutation D394A
    Promoter
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158798-plasmid-map-sequence-id-309523

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  6. pDRFLIP38

    Plasmid
    #65516
    Purpose
    FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.
    Depositor
    Wolf Frommer
    Article
    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755
    Type
    Empty backbone
    Use
    Tags
    6His-edCit-attR1 and attR2-edCer-cMyc
    Expression
    Yeast
    Mutation
    Promoter
    pPMA1
    Available since
    Oct. 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    65516-plasmid-map-sequence-id-111426

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  8. pPP384

    Plasmid
    #158799
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-3(D413A) in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Article
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    dCasPhi-3 (D413A)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158799-plasmid-map-sequence-id-309569

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  9. pFLIP38

    Plasmid
    #65616
    Purpose
    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.
    Depositor
    Wolf Frommer
    Article
    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755
    Type
    Empty backbone
    Use
    Tags
    6His-edCit-attR1 and attR2-edCer-cMyc
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    June 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    65616-plasmid-map-sequence-id-111417

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  13. p3805

    Plasmid
    #198147
    Purpose
    Mammalian expression of LAMP1-miniSOG
    Depositor
    Michael Hensel
    Insert
    PCMV::LAMP1::miniSOG::mCherry (LAMP1 Human)
    Use
    Tags
    miniSOG-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    198147-plasmid-map-sequence-id-388090

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  14. p3806

    Plasmid
    #198148
    Purpose
    Mammalian expression of LAMP1-miniSOG
    Depositor
    Michael Hensel
    Insert
    PCMV::LAMP1::tdminiSOG::mCherry (LAMP1 Human)
    Use
    Tags
    tdminiSOG-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    198148-plasmid-map-sequence-id-388049

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  17. P38m

    Plasmid
    #120948
    Purpose
    MoClo golden gate assembly AB part for pTra* (improved pTra from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    pTra* from Scott and Hasty 2016
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120948-plasmid-map-sequence-id-236376

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  19. pWJPrP38

    Plasmid
    #22110
    Depositor
    Susan Lindquist
    Article
    Jackson et al Neuron. 2009 Aug 27. 63(4):438-50.
    Insert
    PrP genomic vector with cloning sites
    Use
    Construction of targeting vector
    Tags
    Expression
    Mutation
    2.2 Kb EcoR1 fragement isolated from pWJPrP34 and…
    Promoter
    Available since
    Oct. 6, 2009
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 20 of 315 results