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pGEX:PGRMC1
Plasmid#136062PurposeBacterial expression of pGEX:PGRMC1 to perform a GST-pulldown assay and identify new proteins that interact with GST-PGRMC1 in the human endometrial stroll cells.DepositorInsertProgesterone receptor membrane component 1 (PGRMC1 Human)
UseTagsGST-PGRMC1ExpressionBacterialMutationPromoterAvailable sinceMay 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMC1s
Plasmid#17969DepositorInserttetR (B)
UseMycobacteria expressionTagsExpressionMutationPromoterAvailable sinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
MC177VSG221RNAi
Plasmid#59730PurposeFor use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.DepositorInsertVSG221 RNAi fragment
UseRNAiTagsExpressionMutationPromoteropposing T7 promotersAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBAD02_CBASS_Escherichia_coli_BIDMC103
Plasmid#224402PurposeFor expression of the type I CBASS system encoded by Escherichia coli BIDMC103DepositorInsertEcCdnF_AGS-C_SLATT6
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
IBMc180
Plasmid#161942PurposeCarries Golden Gate substitution insert for acVHH-assisted bisection mappingDepositorInsertpSB1A3-BbsI-10aa-acVHH-T-cat-PBAD(SapI)-RiboJ-BCD22-acVHH-10aa-SapI
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc120
Plasmid#161950PurposeContains elements for building reporter plasmid for IBM. Built upon pSB4A3. ECF20 reporter plasmidDepositorInsertpSB4A3-PECF20-B30-mScarlet-I-B15
UseTagsExpressionBacterialMutationPromoterPECF20Available sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
MC1R-Tango
Plasmid#66427PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertMC1R (MC1R Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
TMC1-TwinStrepII
Plasmid#83267PurposeExpression of tagged protein in mammalian cellsDepositorInsertTMC1 (Tmc1 Mouse)
UseTagsTwin Strep Tag IIExpressionMammalianMutationPromoterCMVAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP-CmTMC1truncated
Plasmid#154908PurposeFor the expression and purification of the truncated TMC1 protein from Chelonia mydas. The protease cleavage site was replaced with the HRV 3C site.DepositorInsertGFP-CmTMC1truncated
UseTags8histidine-EGFPExpressionInsectMutationTruncations of the residues 1-243 and 843-969PromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 PSMC1
Plasmid#67087PurposeStandard for cell-free expression benchmarking.DepositorInsertPSMC1 (PSMC1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationE275G, I287V and R294GPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSGE_MC19_Trl-2xUAS_dCP
Plasmid#71170PurposeLuciferase reporterDepositorInsertMC19_Trl-2xUAS_dCP
UseLuciferaseTagsluciferaseExpressionInsectMutationPromoterDSCP Drosophila synthetic core promoterAvailable sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ammC1
Plasmid#167816PurposeExpresses His6-tagged AmmC1 in E. coliDepositorInsertAmmC1
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-PGRMC1-1-54
Plasmid#173472PurposePGRMC1 lacking the C-terminal region downstream of the transmembrane domainDepositorInsertPGRMC1 (PGRMC1 Human)
UseTagsHAExpressionMammalianMutationDeleted for amino acids 55-194PromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA 3 5' cMyc SMC1 wt
Plasmid#32363DepositorInsertSMC1A (SMC1A Human)
UseTagsc-MycExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-57: SMC1A-mEGFP
Plasmid#114406PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human SMC1ADepositorInsertSMC1A Homology Arms with linker-mEGFP (SMC1A Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceSept. 4, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pCAG1.1-Armc12/FLAG
Plasmid#180494PurposeExpression vector of mouse armadillo repeat containing 12 (Armc12) tagged with FLAG at C-terminus. See Fig.4D of Shimada, K. et al. Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021.DepositorInsertarmadillo repeat containing 12 (Armc12 Mouse)
UseTagsFLAG tagExpressionMammalianMutationPromoterCAG promoterAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-PGRMC1-21-195
Plasmid#173473PurposePGRMC1 lacking the N-terminal region upstream of the transmembrane regionDepositorInsertPGRMC1 (PGRMC1 Human)
UseTagsHAExpressionMammalianMutationDeleted for amino acids 1-20PromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHKMC1: Empty sgRNA for Cloning
Plasmid#67720PurposeEmpty vector for cloning sgRNA. NotI and BamHI for cloning sgRNA. PU6 driven sgRNA.DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterPU6Available sinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only
