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Plasmid#139505PurposeAAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.DepositorHas ServiceAAV8 and AAV9Insertparapinopsin
UseAAVTagsVenus tag following 3x Ala linkerExpressionMammalianMutationPromoterEf1aAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-PPO-Venus
Plasmid#139504PurposeAAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the CAG promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.DepositorHas ServiceAAV5Insertparapinopsin
UseAAVTagsVenus tag following 3x Ala linkerExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-OMOR-eYFP
Plasmid#66847PurposeExpresses Opto-MOR in a Cre dependent mannerDepositorInsertOpto Mu opioid receptor
UseAAV and Cre/LoxTagseYFPExpressionMammalianMutationPromoterEf1aAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PPO-Venus
Plasmid#139502PurposeExpresses Venus-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.DepositorInsertparapinopsin
UseTagsVenus tag following 3x Ala linkerExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PPO-mScarlet
Plasmid#139503PurposeExpresses mScarlet-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.DepositorInsertparapinopsin
UseTagsmScarlet following Gly-Gly-Ser linkerExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mScarlet-gamma9
Plasmid#140097PurposeExpresses an mScarlet-tagged gamma9 subunit for visualizing G beta-gamma translocationDepositorInsertGNGT2
UseTagsN-terminal mScarlet tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CRY2-CreN
Plasmid#75268PurposeThe second half of the photo-activatable cre recombinaseDepositorInsertsecond half of the cre recombinase fused to CRY2 (cryptochrome 2) that has absorbed blue light (Kennedy et al., 2010)
UseAAVTagsExpressionMutationPromoterAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CIBN-CreC
Plasmid#75267PurposeThe first half of the photo-activatable cre recombinaseDepositorInsertN-terminal portion of the CIB1 transcription factor
UseAAVTagsExpressionMutationPromoterAvailable sinceOct. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-optoB2-SS-YFP
Plasmid#75270PurposeArrestin independent version of optoB2 with serine mutations at S362A,S363GDepositorInsertArrestin independent version of optoB2
UseTagseGFPExpressionMammalianMutationserine mutations at S362A,S363GPromoterAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-optoB2-LYY-YFP
Plasmid#75271PurposeG-protein independent version of optoB2 with point mutations at L72F,Y136G,Y224ADepositorInsertG-protein independent version of optoB2
UseTagseYFPExpressionMammalianMutationpoint mutations at L72F,Y136G,Y224APromoterAvailable sinceOct. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-PPO-Venus (AAV9)
Viral Prep#139505-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-Ef1a-DIO-PPO-Venus (#139505). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO-PPO-Venus plasmid DNA. EF1a-driven, Cre-dependent expression of Venus-tagged parapinopsin for spatiotemporal control of inhibitory GPCR signaling cascades. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsVenus (Cre-dependent)Available sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-PPO-Venus (AAV5)
Viral Prep#139504-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-CAG-DIO-PPO-Venus (#139504). In addition to the viral particles, you will also receive purified pAAV-CAG-DIO-PPO-Venus plasmid DNA. CAG-driven, Cre-dependent expression of Venus-tagged parapinopsin. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsVenusAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-PPO-Venus (AAV8)
Viral Prep#139505-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-Ef1a-DIO-PPO-Venus (#139505). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO-PPO-Venus plasmid DNA. EF1a-driven, Cre-dependent expression of Venus-tagged parapinopsin for spatiotemporal control of inhibitory GPCR signaling cascades. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsVenus (Cre-dependent)Available sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-CamKII-TRPV1-P2A-mCherry
Plasmid#200829PurposeTransduce excitatory neurons with AAV to express TRPV1 along with an mCherry fluorescence reporterDepositorInsertTRPV1-P2A-mCherry (Trpv1 Rat)
UseAAVTagsExpressionMammalianMutationPromoterCamKIIAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-tdTomato
Plasmid#128434PurposeExpresses tdTomato under control of eukaryotic Ef1a promoter after Flp-dependent recombinationDepositorHas ServiceAAV1InserttdTomato
UseAAVTagsExpressionMammalianMutationPromoterEf1aAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-ChrimsonR-tdTomato
Plasmid#171027PurposeExpresses ChrimsonR-tdTomato fusion after Flp-dependent recombinationDepositorHas ServiceAAV1 and AAV9InsertChrimsonR-tdTomato
UseAAVTagsChrimsonR is fused to tdTomatoExpressionMutationPromoterEf1aAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-Syn-TRPV1-P2A-DsRed
Plasmid#200830PurposeTransduce all neuron types with AAV to express TRPV1 along with a DsRed fluorescence reporterDepositorInsertTRPV1-P2A-DsRed (Trpv1 Rat)
UseAAVTagsExpressionMammalianMutationPromoterSynAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV_NOPlight1
Plasmid#195578PurposeExpresses NOPLight1 in mammalian cellsDepositorInsertNOPLight1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-GCaMP6f
Plasmid#128315PurposeExpresses GCaMP6f under control of eukaryotic Ef1a promoter after Flp-dependent recombination.DepositorHas ServiceAAV1InsertGCaMP6f
UseAAVTagsHis TagExpressionMammalianMutationPromoterEf1aAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-Syn-DIO-TRPV1-P2A-mCherry
Plasmid#200831PurposeIn the presence of Cre, drives neuron-specific expression of TRPV1 with fluorescence reporter mCherryDepositorInsertTRPV1-P2A-mCherry (Trpv1 Rat)
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only