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Plasmid Kit#1000000213Purpose'Calling Cards' technology using self-reporting transposons (SRTs) enables the identification of DNA-protein interactions through RNA sequencing.DepositorAvailable sinceFeb. 16, 2023AvailabilityAcademic Institutions and Nonprofits only
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Human Transporter Knockout library
Pooled Library#213695PurposeNext generation transporter-focused CRISPR/Cas9 library targeting transmembrane transporters to test the impact of transporters on biological processes.DepositorSpeciesHomo sapiensUseCRISPR and LentiviralAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
Human Transporter Knockout Library with UMIs
Pooled Library#221409PurposegRNA library to test the impact of transporters on biological processesDepositorSpeciesHomo sapiensUseCRISPR and LentiviralAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
Human ABC Transporter Knockout Sub-library with UMIs
Pooled Library#221410PurposegRNA library to test the impact of transporters on biological processes (subset: ABC transporters and controls)DepositorSpeciesHomo sapiensUseCRISPR and LentiviralAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
MKC176_EPOR(2A-synEPOR)
Plasmid#232414PurposeAAV production plasmid for EPOR(synEPOR) vector from Figs. 3-5 that mediates HDR at EPOR locus using EPOR gRNA. synEPOR is codon diverged to prevent premature recombination with EPOR locus.DepositorInsertTruncated Erythropoietin Receptor (EPOR Human)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE311B
Plasmid#120418PurposeExpresses Lambda Beta and a dominant negative MutL E32K allele, controlled by XylS-Pm expression system for high precision and efficient MAGE experiments at 37°C. RSF1010 Ori; Broad host-range; KanRDepositorArticleInsertsMutL E32K
Lambda Beta
XylS
UseTagsExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterPmAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-3
Plasmid#72678PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Kan resistance marker.DepositorInsertmutL E32K
UseTagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-4
Plasmid#72679PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Cam resistance marker.DepositorInsertmutL E32K
UseTagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE312B
Plasmid#128969PurposeBroad-host-range KanR plasmid expressing PapRecT and EcMutL_E32K with Toluic acid inductionDepositorInsertsPapRecT
mutL E32K
UseTagsExpressionBacterialMutationE32KPromoterxylS-PmAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pORF1-2
Plasmid#206024PurposeAn expression vector for mouse LINE1 ORF1-2DepositorInsertORF1-2 derived from mouse LINE1
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pORF2
Plasmid#206026PurposeAn expression vector for mouse LINE1 ORF2DepositorInsertORF2 derived from mouse LINE1
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pORI28
Plasmid#71595PurposeEC1000 containing pORI28. Used as the source of plasmid pORI28. With pTRK669, part of a system for chromosomal integration in gram positive bacteria.DepositorTypeEmpty backboneUseIntegration vector for bacteriaTagsExpressionMutationPromoterAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-2
Plasmid#72677PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Ap resistance marker.DepositorInsertmutL E32K
UseTagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterpL promoterAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
yeast_DualReporter
Plasmid#127546PurposeA dual reporter vector modified from Sharon et al (Nature Biotech, 2012) containing Ura3 and Nat1 selectable markers, a constant background RFP (TEF2::mCherry), and a variable YFP (pTpA+MCS::yEVenus).DepositorInsertsUseSynthetic BiologyTagsExpressionYeastMutationPromoterTEF, TEF2, URA3, bla (E. coli), and pTpA+MCSAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pORE303N
Plasmid#194438PurposeCRISPR, Cas9 driven by double 35S, nptII for plant selection, KpnI site for gRNA insertionDepositorInsertCas9
UseCRISPRTagsExpressionPlantMutationPromoter2x35SAvailable sinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ddo_porcupine-1
Plasmid#107631PurposeIn situ hybridization and RNAi studies in planariansDepositorInsertporcupine-1
UseRNAi; In situ hybridizationTagsExpressionMutationPromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
Reporter-1
Plasmid#180218PurposeSurrogate reporter plasmid for the assay of targeted RNA editingDepositorInsertSurrogate reporter
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-1
Plasmid#72680PurposeSame as pORTMAGE-2, but with a non-optimized RBS before mutant MutL, thus lower level of expression of the enzyme.DepositorInsertmutL E32K
UseTagsnoneExpressionBacterialMutationlower strength RBS before mutL E32K allele than i…PromoterAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pORFE0010
Plasmid#112074Purpose3xmVenus module CTDepositorInsert3xmVenus
UseTagsExpressionPlantMutationSer to Thr at amino acid 63 and Phe to Leu at pos…PromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
Reporter-2
Plasmid#180219PurposeSurrogate reporter plasmid for the assay of targeted RNA editingDepositorInsertSurrogate reporter
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only