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hSyn-GEM
Plasmid#187648PurposeExpresses the rheological probe PfV-HaloTag-SNAP-tag (GEM-HaloTag-SNAP-tag) under control of human synapsin promoter.DepositorInsertPfV
UseAAVTagsHaloTag and SNAP-tagExpressionMammalianMutationPromoterSynapsinAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
mOtop3_pGEMHE
Plasmid#114676PurposeThis construct was used to express mouse Otop3 in Xenopus laevis oocytesDepositorInsertOtopetrin 3 (Otop3 Mouse)
UseFor generating the mrna, which can highly express…TagsExpressionMutationPromoterT7Available sinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEMS-II
Plasmid#204765PurposeGEMS sensor system (pCMV-EGFP-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577)DepositorInsertEGFP-m6Alinker-DHFR
UseTagsExpressionMutationmutated all RACs in EGFP to RGC/RCCPromoterCMVAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT-GEM(2)
Plasmid#62894PurposeTrojan-Gal4 Expression Module in Phase 2. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exonsDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEmCherry2
Plasmid#118413PurposeRed fluorescent reporter for assessing CRISPR gRNA activityDepositorInsertmCherry
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT-GEM(0)
Plasmid#62891PurposeTrojan-Gal4 Expression Module in Phase 0. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exonsDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
mOtop2_pGEMHE
Plasmid#114675PurposeThis construct was used to express mouse Otop2 in Xenopus laevis oocytesDepositorInsertOtopetrin 2 (Otop2 Mouse)
UseFor generating the mrna, which can highly express…TagsExpressionMutationPromoterT7Available sinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT-GEM(1)
Plasmid#62893PurposeTrojan-Gal4 Expression Module in Phase 1. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exonsDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSGEMKv10.1
Plasmid#85704PurposeXenopus laevis oocyte expression of Kv10.1DepositorInsertKCNH1 (KCNH1 Human)
UseXenopus expressionTagsExpressionMutationPromoterAvailable sinceDec. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEMABC
Plasmid#45398DepositorUseTagsExpressionBacterialMutationPromoterNone and T7Available sinceJune 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
mOtop1_pGEMHE
Plasmid#114674PurposeThis construct was used to express mouse Otop1 in Xenopus laevis oocytesDepositorInsertOtopetrin 1 (Otop1 Mouse)
UseFor generating the mrna, which can highly express…TagsExpressionMutationSNP (rs49325632): Glycine 396 to AlaninePromoterT7Available sinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM-2t
Plasmid#159752PurposeCRISPR/Cas9 genome editing in plants; a template for PCR-derived fragments used in the assembly of polycistronic transcripts, where sgRNAs are separated by an Arabidopsis alanine tRNA.DepositorArticleInsertsgRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
CFP_Gem_pcDNA4_HisMaxC
Plasmid#41653DepositorInsertGem, GTP binding proteins (RGK) (GEM Human)
UseTagsCFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEMHEZFCNGA5
Plasmid#69861Purposeoocyte expression vector containing ZFCNGA5DepositorInsertZFCNGA5 (cnga5 Zebrafish)
UseXenopus oocyte expressionTagsExpressionMutationnonePromoterT7Available sinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEM_ΔndhD2.km
Plasmid#185532Purposea pGEM-T easy vector containing the kanamycin resistance cassette flanked by the two regions for the double homologous recombination on the ndhD2 locusDepositorInsertnptII gene flanked by ndhD2 upstream and downstream regions
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only
