All-in-One CRISPRko system with a guide RNA that targets GART gene

Bacterial expression for structure determination; may not be full ORF

Bacterial expression for structure determination; may not be full ORF

expresses human GART protein with a C-terminal 2XStrep affinity tag

Expresses human GART-GFP fusion in mammalian cells

Expresses human GART-mCherry fusion in mammalian cells

Ready-to-use Lentiviral Prep particles produced from Human sgRNA library Brunello in lentiCRISPRv2 (#73179). In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiCRISPRv2 plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR screening in human cells. This backbone contains SpCas9 and unique gRNAs, and can be used to make edits across 19,114 genes in the human genome. In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiCRISPRv2.

Ready-to-use Lentiviral Prep particles produced from Human sgRNA library Brunello in lentiGuide-Puro (#73178). In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiGuide-Puro plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR screening in human cells. Use on cells that are stably expressing Cas9 to make edits across 19,114 genes in the human genome.

Ready-to-use Lentiviral Prep particles produced from Mouse sgRNA library Brie in lentiGuide-Puro (#73633). In addition to the viral particles, you will also receive purified Mouse sgRNA library Brie in lentiGuide-Puro plasmid DNA. <p>&lt;p&gt;Ready-to-use lentiviral pooled library for CRISPR screening in mouse cells. Use on cells that are stably expressing Cas9 to make edits across 19,674 genes in the mouse genome.&lt;/p&gt;</p>

overespression of Flp step-arrest mutant in yeast, LYS2 selection

single copy recombinase yeast expression plasmid

pEF-HA-NLS-CloningSite-mCherry-puroR

pEF-HA-PYL1-NLSminus-CloningSite-mCherry-puroR

pEF-HA-3xNES-CloningSite-mCherry-puroR

overespression of Flp step-arrest mutant in yeast, URA selection

Met15 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.

overespression of Flp step-arrest mutant in yeast, TRP1 selection

ADE2 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.

Leu2 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.