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Plasmid Kit#1000000036PurposeGolden Gate cloning system for rapid assembly of plant transformation constructs; Uses 6 types of pre-cloned modules: promoter, N- and C-terminal tags, coding sequence, terminator, resistance cassetteDepositorAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only
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pGoldenGreenGate-M (pGGG-M)
Plasmid#165422PurposeA Golden Gate (MoClo) compatible Level 2 acceptor binary vector based on pGreenII (Hellens et al., 2000).TypeEmpty backboneUseSynthetic Biology; Level 2 golden gate (moclo) du…TagsExpressionMutationPromoterContain GG cassette and MCS with LacZ for blue/wh…Available sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
GreenGate 2.0 Toolkit
Plasmid Kit#1000000241PurposeThe GreenGate 2.0 (GG2.0) toolkit enables the assembly and stacking of complex transcriptional units with the GreenGate Cloning System. It is backwards compatible with most existing GreenGate modules.DepositorAvailable sinceApril 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
SapTrap-SEC kit for C. elegans genome engineering
Plasmid Kit#1000000109PurposeBuild sgRNA and homologous repair templates that incorporate self-excising drug selection cassette (SEC), using SapTrap cloning method. Addition of AAG junction.DepositorAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
MoClo Plant Parts III: Transformation & Genome Engineering Kit
Plasmid Kit#1000000236PurposeThe MoClo Plant Parts III: Transformation & Genome Engineering Kit contains 94 Level 0 parts, compatible with the Phytobrick/MoClo system for golden gate assembly of plant transformation constructs.DepositorAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
H3K9me3Probe_FullLength
Plasmid#186463PurposeExpresses GFP tagged H3K9me3 recognition motif in mammalian cells.DepositorInsertdimeric CDY1 (CDY1 Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-5'-ENGRAM
Plasmid#179157PurposepiggyBac plasmid for 5' ENGRAM recorderDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterminPAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
LENG
Plasmid#111162PurposemiR-E (miR-30 variant)-based RNAi with GFP reporterDepositorTypeEmpty backboneUseRetroviralTagsGFPExpressionMutationPromoterAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-3-FT-ENGRAM
Plasmid#179158PurposepiggyBac plasmid for 3'-FT ENGRAM recorderDepositorInsertCsy4
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterminPAvailable sinceDec. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVE2_20_p40S_QfDBD_VP16AD_pFullLength_pcbAB_DsRed
Plasmid#154242PurposeEncodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with full lenght promoter pcbABDepositorInsertp40S:QfDBD-VP16AD-GFP-NLS
UseSynthetic Biology; Fungal expressionTagsGFP-NLS; DsRed-SKLExpressionBacterialMutationPromoterp40S (AN0465 A. nidulans)Available sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVE2_19_p40S_QfDBD_VP16AD_pFullLength_pcbC_DsRed
Plasmid#154241PurposeEncodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with full lenght promoter pcbCDepositorInsertp40S:QfDBD-VP16AD-GFP-NLS
UseSynthetic Biology; Fungal expressionTagsGFP-NLS; DsRed-SKLExpressionBacterialMutationPromoterp40S (AN0465 A. nidulans)Available sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGoldenGate-SE9
Plasmid#47677PurposeBinary vector for Arabidopsis transformation with BsaI Golden Gate sites and Kanamycin selection.DepositorTypeEmpty backboneUseArabidopsis transformationTagsExpressionMutationPromoterAvailable sinceSept. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGoldenGate-MCY2
Plasmid#47679PurposeBinary vector for Arabidopsis transformation with SapI Golden Gate sites and mCherry seed selectionDepositorTypeEmpty backboneUseArabidopsis transformationTagsExpressionMutationPromoterAvailable sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
PCBP1_Full-length_pGEX
Plasmid#135108PurposeRecombinant expression of PCBP1 with dual-affinity tagDepositorInsertPCBP1 (PCBP1 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
SNRPA_Full-length_pGEX
Plasmid#135134PurposeRecombinant expression of SNRPA with dual-affinity tagDepositorInsertSNRPA (SNRPA Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceFeb. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGoldenGate-SE7
Plasmid#47676PurposeBinary vector for Arabidopsis transformation with BsaI Golden Gate sites and mCherry seed selection.DepositorTypeEmpty backboneUseArabidopsis transformationTagsExpressionMutationPromoterAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GoldenGate
Plasmid#47388PurposeExpression plasmid for TALE proteins compatible with GoldenGate cloning systemDepositorTypeEmpty backboneUseTALENTagsFlag, HA, and SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
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RBM45_Full-length_pGEX
Plasmid#135127PurposeRecombinant expression of RBM45 with dual-affinity tagDepositorInsertRBM45 (RBM45 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
NOVA1_Full-length_pGEX
Plasmid#135147PurposeRecombinant expression of NOVA1 with dual-affinity tagDepositorInsertNOVA1 (NOVA1 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceAug. 26, 2022AvailabilityAcademic Institutions and Nonprofits only