1. BPK1520

   Plasmid

   #65777

   Purpose

   Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   SpCas9 gRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. BPK1520_blastR

   Plasmid

   #175289

   Purpose

   Human expression plasmid for SpCas9 sgRNA with blasticidin co-selection

   Depositor

   Ronald Cohn

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/U6

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. BPK1520_puroR

   Plasmid

   #173901

   Purpose

   SpCas9 guide cloning vector derived from BPK1520 that allows for puromycin-based selection

   Depositor

   Ronald Cohn

   Article

   Erwood et al Genome Res. 2019 Dec;29(12):2010-2019. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/U6

   Available since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pU6 SgRNA RBM20

   Plasmid

   #162735

   Purpose

   Mammalian expression

   Depositor

   Amit Choudhary

   Article

   Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

   Insert

   Spacer sequence for RBM20
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pU6 SgRNA GAPDH

   Plasmid

   #162736

   Purpose

   Mammalian expression

   Depositor

   Amit Choudhary

   Article

   Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

   Insert

   Spacer sequence for GAPDH
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDmActin5c

   Plasmid

   #165777

   Purpose

   Drosophila Actin 5C promoter

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Actin5c
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. BPK1520-LRRK2-G2019S-ng

   Plasmid

   #180436

   Purpose

   Transiently expressing a nicking sgRNA to facilitate the introduction of LRRK2-G2019S mutation in PE3 approach

   Depositor

   Frank Soldner

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   ncRNA targeting LRRK2-G2019S (LRRK2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBPK1520-SNCA-A53T-ng

   Plasmid

   #181739

   Purpose

   Transiently expressing a nicking sgRNA to facilitate the introduction of SNCA-A53T mutation in PE3 approach

   Depositor

   Dirk Hockemeyer

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   Prime editing ngRNA for SNCA-A53T (SNCA Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBPK1520-HEK3-CTTins-ng

   Plasmid

   #180019

   Purpose

   Transiently expressing a nicking sgRNA to facilitate the introduction of HEK3 CTT insertion in PE3 approach

   Depositor

   Dirk Hockemeyer

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   Prime editing ngRNA for HEK3-CTTins (EPHA8 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U7

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBPK1520-SNCA-A30P-ng

   Plasmid

   #180018

   Purpose

   Transiently expressing a nicking sgRNA to facilitate the introduction of SNCA-A30P mutation in PE3 approach

   Depositor

   Dirk Hockemeyer

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   Prime editing ngRNA for SNCA-A30P (SNCA Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. YM138

    Bacterial Strain

    #61953

    Purpose

    C43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. cysE gene deleted.

    Depositor

    Toshio Iwasaki, Robert Gennis

    Article

    Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

    Bacterial Resistance

    None

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

11. RF10

    Bacterial Strain

    #62076

    Purpose

    BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. lysA genes deleted.

    Depositor

    Toshio Iwasaki, Robert Gennis

    Article

    Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

    Bacterial Resistance

    None

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

12. RF11

    Bacterial Strain

    #61961

    Purpose

    C43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. metA gene deleted

    Depositor

    Toshio Iwasaki, Robert Gennis

    Article

    Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

    Bacterial Resistance

    None

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

13. RF1

    Bacterial Strain

    #61962

    Purpose

    BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. glyA gene deleted

    Depositor

    Toshio Iwasaki, Robert Gennis

    Article

    Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

    Bacterial Resistance

    None

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

14. RF6

    Bacterial Strain

    #62074

    Purpose

    BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. proC gene deleted.

    Depositor

    Toshio Iwasaki, Robert Gennis

    Article

    Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

    Bacterial Resistance

    None

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

15. RF2

    Bacterial Strain

    #62070

    Purpose

    BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. thrC gene deleted

    Depositor

    Toshio Iwasaki, Robert Gennis

    Article

    Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

    Bacterial Resistance

    None

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

16. RF8

    Bacterial Strain

    #62075

    Purpose

    BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. asnA asnB genes deleted.

    Depositor

    Toshio Iwasaki, Robert Gennis

    Article

    Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

    Bacterial Resistance

    None

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

17. MS1

    Bacterial Strain

    #61960

    Purpose

    C43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. cysE hisG genes deleted

    Depositor

    Toshio Iwasaki, Robert Gennis

    Article

    Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

    Bacterial Resistance

    None

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

18. RF4

    Bacterial Strain

    #62072

    Purpose

    BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB genes deleted.

    Depositor

    Toshio Iwasaki, Robert Gennis

    Article

    Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

    Bacterial Resistance

    None

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

19. RF12

    Bacterial Strain

    #62077

    Purpose

    BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. trpA trpB genes deleted.

    Depositor

    Toshio Iwasaki, Robert Gennis

    Article

    Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

    Bacterial Resistance

    None

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only