1. p426 72Q GPD

   Plasmid

   #1183

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 72Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

These results may also be relevant.

1. mCherry-RanV19

   Plasmid

   #118316

   Purpose

   Expression of human Ran

   Depositor

   Philip Stork

   Article

   Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

   Insert

   RanV19 (RAN Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. hHSF1 K80Q

   Plasmid

   #118351

   Purpose

   Plasmid expresses hHSF1 with K80 in DBD mutated to Q, which abolish DNA-binding activity of HSF1.

   Depositor

   Lea Sistonen

   Article

   Westerheide et al Science. 2009 Feb 20. 323(5917):1063-6.

   Insert

   hHSF1 (HSF1 Human)
   

   Use

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   K80Q

   Promoter

   Available since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. hMEF2A WT

   Plasmid

   #118354

   Purpose

   This plasmid expresses human MEF2A-1 WT.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

   Insert

   hMEF2A (MEF2A Human)
   

   Use

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. hGATA-1 WT

   Plasmid

   #118352

   Purpose

   This plasmid expresses human GATA-1 WT.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

   Insert

   hGATA-1 (GATA1 Human)
   

   Use

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. KAT14-pDEST10

   Plasmid

   #118382

   Purpose

   HIS-tagged insect cell expression vector with KAT14.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Insert

   KAT14 (KAT14 Human)
   

   Use

   Tags

   HIS

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   May 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. 2Flag-pDEST-C

   Plasmid

   #118372

   Purpose

   Gateway compatible mammalian expression vector with C-terminal 2Flag to tag proteins of interest.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Type

   Empty backbone

   Use

   Destination

   Tags

   2Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 2HA-pDEST-C

   Plasmid

   #118374

   Purpose

   Gateway compatible mammalian expression vector with C-terminal 2HA to tag proteins of interest.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Type

   Empty backbone

   Use

   Destination

   Tags

   2HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLenti-X1-hygro-mCherry-RAMP4

   Plasmid

   #118391

   Purpose

   stable lentiviral expression of cDNA

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   RAMP4 (SERP1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   EIF-1a promoter

   Available since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Flag-GFP11-pDEST-C

   Plasmid

   #118367

   Purpose

   Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Type

   Empty backbone

   Use

   Destination

   Tags

   GFP11-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HA-GFP1-10-pDEST-N

    Plasmid

    #118368

    Purpose

    Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Type

    Empty backbone

    Use

    Destination

    Tags

    HA-GFP1-10

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Flag-GFP11-pDEST-N

    Plasmid

    #118366

    Purpose

    Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Type

    Empty backbone

    Use

    Destination

    Tags

    GFP11-Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. HA-BirA*-pDEST-N-pcDNA5/FRT/TO

    Plasmid

    #118375

    Purpose

    Expression vector for BioID in mammalian system. Gateway compatible destination vector to generate stable, inducible cell line expressing N-terminally tagged HABirA* and protein of interest.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Type

    Empty backbone

    Use

    Destination

    Tags

    HA-BirA*

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. HA-GFP1-10-pDEST-C

    Plasmid

    #118369

    Purpose

    Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Type

    Empty backbone

    Use

    Destination

    Tags

    HA-GFP1-10

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-Xiap-Myc

    Plasmid

    #11833

    Depositor

    Guy Salvesen

    Article

    Takahashi et al J Biol Chem. 1998 Apr 3. 273(14):7787-90

    Insert

    XIAP (XIAP Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. hHSF1 K80R

    Plasmid

    #118350

    Purpose

    Plasmid expresses hHSF1 with K80 in DBD mutated to R.

    Depositor

    Lea Sistonen

    Article

    Westerheide et al Science. 2009 Feb 20. 323(5917):1063-6.

    Insert

    hHSF1 (HSF1 Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    K80R

    Promoter

    Available since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-Xiap-Myc K322/328R

    Plasmid

    #11834

    Depositor

    Guy Salvesen

    Insert

    XIAP K322/328R (XIAP Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    K322/328R

    Promoter

    Available since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

17. 2HA-pDEST-N

    Plasmid

    #118373

    Purpose

    Gateway compatible mammalian expression vector with N-terminal 2HA to tag proteins of interest.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Type

    Empty backbone

    Use

    Destination

    Tags

    2HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pENTR-NLS-actin-R62D

    Plasmid

    #118381

    Purpose

    Gateway compatible donor vector with nuclear localization signal (NLS) attached to actin-R62D.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Insert

    actin beta (ACTB Human)
    

    Use

    Donor-plasmid

    Tags

    Expression

    Mutation

    NLS and R62D

    Promoter

    Available since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. hHSF4b WT

    Plasmid

    #118356

    Purpose

    This plasmid expresses human HSF4b WT.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hHSF4b (HSF4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p221z-CAS9p-TagRFP-t35s

    Plasmid

    #118386

    Purpose

    Entry clone containing CAS9p-TagRFP and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

    Insert

    CAS9p-TagRFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge