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Showing: 1 - 50 of 89 results

  13. pSPNprM-hp

    Plasmid
    #48120
    Purpose
    Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein NprM under control of the xylose inducible promoter
    Depositor
    Dieter Jahn
    Article
    Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):
    Insert
    signal peptide of NprM
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 11, 2013
    Availability
    Academic Institutions and Nonprofits only
    48120-plasmid-map-sequence-id-69946

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  15. pDONR221-SPNS1_STOP

    Plasmid
    #161439
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SPNS1 (SPNS1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161439-plasmid-map-sequence-id-355837

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  16. pDONR221-SPNS3_STOP

    Plasmid
    #161451
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SPNS3 (SPNS3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161451-plasmid-map-sequence-id-355838

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  17. pDONR221-SPNS2_STOP

    Plasmid
    #161473
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SPNS2 (SPNS2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161473-plasmid-map-sequence-id-355839

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  18. M-SPn-VP64

    Plasmid
    #48674
    Purpose
    Mammalian SP-VP64 nuclease-null Cas9 activator expression, human optimized
    Depositor
    George Church
    Article
    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n
    Insert
    Cas9-VP64, nuclease-null
    Use
    CRISPR
    Tags
    NLS and VP64
    Expression
    Mutation
    nuclease-null
    Promoter
    CMV
    Available since
    Dec. 9, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. SPN-bio-His

    Plasmid
    #51829
    Purpose
    Expresses full-length Leukosialin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    SPN (SPN Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51829-plasmid-map-sequence-id-79665

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  29. pFHMSPN-avi-TEV-LIC V

    Plasmid
    #220885
    Purpose
    Donor vector to generate recombinant baculovirus by site-specific transposition in an E. coli host.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Baculovirus expresssion
    Tags
    Honeybee melittin signal followed by His, avi and…
    Expression
    Mutation
    Promoter
    Available since
    June 20, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    220885-plasmid-map-sequence-id-436698

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  33. pMXs-IRES-blast 3xFlag-SPNS1(E164K)

    Plasmid
    #198569
    Purpose
    SPNS1(E164K) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector
    Depositor
    Monther Abu-Remaileh
    Article
    Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi
    Insert
    SPNS1(E164K) with n-terminal 3xFlag (SPNS1 Human)
    Use
    Retroviral
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    changed glutamic acid E164 to lysine (E164K)
    Promoter
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    198569-plasmid-map-sequence-id-438870

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  35. pMXs-IRES-blast GFP-SPNS1(E164K)

    Plasmid
    #198572
    Purpose
    SPNS1(E164K) codon-optimized orf with n-terminal 3xFlag GFP in pMXs-IRES-Blast vector
    Depositor
    Monther Abu-Remaileh
    Article
    Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi
    Insert
    SPNS1(E164K) with n-terminal 3xFlag-GFP fusion (SPNS1 Human)
    Use
    Retroviral
    Tags
    3xFlag-GFP
    Expression
    Mammalian
    Mutation
    changed glutamic acid E164 to lysine (E164K)
    Promoter
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    198572-plasmid-map-sequence-id-438878

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  40. pCM1.120

    Plasmid
    #19600
    Depositor
    Geraldine Seydoux
    Insert
    spn-4 prom in PDONRP4P1R
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Nov. 10, 2008
    Availability
    Academic Institutions and Nonprofits only
    19600-plasmid-map-sequence-id-9234

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  41. pCM1.120

    Plasmid
    #19600
    Depositor
    Geraldine Seydoux
    Insert
    spn-4 prom in PDONRP4P1R
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Nov. 10, 2008
    Availability
    Academic Institutions and Nonprofits only
    19600-plasmid-map-sequence-id-9234

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  43. pDEAF1.2.2-gDNA

    Plasmid
    #113799
    Purpose
    CRISPR plasmid for expression of Cas9 nicakse (D10A) and gRNA targeting human transcription factor DEAF1
    Depositor
    Kevin White
    Insert
    DEAF1 (DEAF1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113799-plasmid-map-sequence-id-230651

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  44. pDEAF1.2.1-gDNA

    Plasmid
    #112449
    Purpose
    CRISPR plasmid for expression of Cas9 nickase (D10A) and gRNA targeting human transcription factor DEAF1
    Depositor
    Kevin White
    Insert
    DEAF1 (DEAF1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112449-plasmid-map-sequence-id-230462

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  46. TFORF1183

    Plasmid
    #143936
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    DEAF1 (DEAF1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    143936-plasmid-map-sequence-id-404598

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  47. avi-eTMPRSS11D S368A

    Plasmid
    #220882
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TMPRSS11D (TMPRSS11D Human)
    Use
    Baculovirus expresssion
    Tags
    Honeybee melittinsignal
    Expression
    Mutation
    S368A
    Promoter
    Polyhedrin
    Available since
    June 20, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    220882-plasmid-map-sequence-id-436699

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  49. pEditC

    Plasmid
    #207532
    Purpose
    Plasmid for cytidine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→CBE:SpnCas9, PEM7→non-specific sgRNA; SmR/SpR
    Depositor
    Pablo Ivan Nikel
    Article
    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.
    Insert
    xylS (cured of BsaI-sites), Pm→CBE:SpnCas9, PEM7→non-specific sgRNA
    Use
    CRISPR; Pseudomonas vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    207532-plasmid-map-sequence-id-409609

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  50. pEditA

    Plasmid
    #207531
    Purpose
    Plasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ ABE:SpnCas9, PEM7→non-specific sgRNA; SmR/SpR
    Depositor
    Pablo Ivan Nikel
    Article
    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.
    Insert
    Plasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ ABE:SpnCas9, PEM7→non-specific sgRNA
    Use
    CRISPR; Pseudomonas vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    207531-plasmid-map-sequence-id-409606

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Showing: 1 - 50 of 89 results