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Pooled Library#163966PurposeLentiviral CRISPR library targets 2,918 mouse metabolic genes with ~18,000 guide RNAs. The library contains ~6 gRNAs per gene.DepositorUseCRISPR and LentiviralAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only
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Mouse metabolism knockout library
Pooled Library#160129PurposeCRISPR library targets mouse metabolic genesDepositorExpressionMammalianSpeciesMus musculusUseCRISPR and LentiviralAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
Human Lipid Droplet and Metabolism Library
Pooled Library#191535PurposeLentiviral CRISPR library targeting 1,196 genes related to lipid droplet biology and cell metabolism.DepositorExpressionMammalianUseCRISPR and LentiviralAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
Mouse sphingolipid metabolism knockout library
Pooled Library#206249PurposeThe sphingolipid metabolism library targets 82 mouse genes in the de novo ceramide and downstream sphingolipid metabolism pathways.DepositorUseCRISPR and LentiviralAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Mouse lipid metabolism knockout library
Pooled Library#206248PurposeThe lipid metabolism library contains guide RNAs against 295 genes targeting mouse genes encoding enzymes that directly impact lipid metabolism.DepositorUseCRISPR and LentiviralAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Human CRISPR Metabolic Gene Knockout Library
Pooled Library#110066PurposeEncodes gRNAs for targeting metabolic genes in CRISPR screens.DepositorUseLentiviralAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
Human Metabolic Gene CRISPRa sgRNA library
Pooled Library#187080PurposeLentiviral CRISPRa library targeting human metabolic genes for activation of gene expressionDepositorExpressionMammalianSpeciesHomo sapiensUseLentiviralAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
Mouse Metabolic Gene CRISPRa sgRNA library
Pooled Library#187081PurposeLentiviral CRISPRa library targeting mouse metabolic genes for activation of gene expressionDepositorExpressionMammalianSpeciesMus musculusUseLentiviralAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ubi:Zebrabow
Plasmid#118219PurposeFor ubiquitous expression of Brainbow in zebrafishDepositorInsertBrainbow 1.0 L
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
UAS:Zebrabow-B
Plasmid#118216PurposeFor broad gal4 inducible expression of Brainbow in zebrafishDepositorInsertBrainbow 1.0 L
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
UAS:Zebrabow-V
Plasmid#118218PurposeFor broad or variegated gal4 inducible expression of Brainbow in zebrafishDepositorInsertBrainbow 1.0 L
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceNov. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEM_ZmaBOP1binsitu
Plasmid#136531PurposeTemplate for generating in situ hybridization probeDepositorInsertZoysia matrella BLADE-ON-PETIOLE homolog1b
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEM_ZmaBOP2binsitu
Plasmid#136532PurposeTemplate for generating in situ hybridization probeDepositorInsertZoysia matrella BLADE-ON-PETIOLE homolog2b
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
Zebrabow-GateDest
Plasmid#118221PurposeFor cloning promoter into a Zebrabow vector with Gateway reaction. Only compatible with single site Gateway. Not compatible with Tol2-kit.DepositorInsertBrainbow 1.0 L - ccdB survival cassette
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
LEA1_APHAV_Chakrabortee pSecTag (pBS0254)
Plasmid#185143PurposeFor the mammalian expression of the nematode (Aphelenchus avenae) protein LEA1_APHAV. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).DepositorInsertLEA1_APHAV
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBbS5c-AcrB/AcrD/AcrFa, ABO_0964
Plasmid#86206PurposeExpresses efflux pump in E. coli to provide resistance to biofuel (low copy)DepositorInsertEfflux pump
UseTagsExpressionBacterialMutationPromoterATCGTTTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAA…Available sinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBbA5k-AcrB/AcrD/AcrFa, ABO_0964
Plasmid#86202PurposeExpresses efflux pump in E. coli to provide resistance to biofuel (medium copy)DepositorInsertEfflux pump
UseTagsExpressionBacterialMutationPromoterATCGTTTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAA…Available sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
LIR-wtloxP-TRE-CAG-Frt-red-puro-3xPA-Frt-GFP(mVenus)-KrasG12D-cMyc-SV40LT-IRES-KRABoff-PA-TRE-loxP257-RIR
Plasmid#67277Purposeinducbile color change and cancer regulation: FlpO recombinase dependent expression of KrasG12D cMyc and SV40 large T antigen with doxycycline inducible downregulation through tetKRAB system.DepositorInsertsfirst casette contain a red fluorescent protein (katushka) linked to puromycin resitance gene through an E2A site
second cassette contains mVENUS-kras-cmyc-SV40LT-KRABoff
UseTagsmTQ2 blue fluorescent and red flourescent gene ka…ExpressionMammalianMutationPromoterCAGAvailable sinceAug. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pMax_GFP
Plasmid#177825PurposeHost Cell Reactivation (HCR) control GFP plasmid (pMax backbone)DepositorInsertGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMax_mOrange
Plasmid#177827PurposeHost Cell Reactivation (HCR) control mOrange plasmid (pMax backbone)DepositorInsertmOrange
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMax_mPlum
Plasmid#177830PurposeHost Cell Reactivation (HCR) control mPlum plasmid (pMax backbone)DepositorInsertmPlum
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMax_ScaI_BFP
Plasmid#177824PurposeSite-specific mutagenesis of tagBFP for the purposes of creating a clean double strand break for the purposes of measuring NHEJ activity via Host Cell Reactivation (HCR).DepositorInserttagBFP
UseTagsExpressionMammalianMutation5' inserted ScaI RE cut sitePromoterCMVAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMax_mOrange_A215C
Plasmid#177828PurposeSite-specific mutagenesis of mOrange for the purposes of creating a site-specific 8-oxo-G:C lesion for the purposes of measuring OGG1 activity via Host Cell Reactivation (HCR).DepositorInsertmOrange_A215C
UseTagsExpressionMammalianMutationA215CPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMax_GFP_C289T
Plasmid#177826PurposeSite-specific mutagenesis of GFP for the purposes of creating a site-specific hypoxanthine lesion for the purposes of measuring MPG activity via Host Cell Reactivation (HCR).DepositorInsertGFP_C289T
UseTagsExpressionMammalianMutationC289TPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMax_mOrange_G299C
Plasmid#177829PurposeSite-specific mutagenesis of mOrange for the purposes of creating a site-specific G:G mispair for the purposes of measuring MMR activity via Host Cell Reactivation (HCR).DepositorInsertmOrange_G299C
UseTagsExpressionMammalianMutationG299CPromoterCMVAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMax_BFP_A191G
Plasmid#177823PurposeSite-specific mutagenesis of tagBFP for the purposes of creating a site-specific U:A mispair for the purposes of measuring UDG activity via Host Cell Reactivation (HCR).DepositorInserttagBFP_A191G
UseTagsExpressionMammalianMutationA191GPromoterCMVAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMax_mPlum_C207G/T208C
Plasmid#177831PurposeSite-specific mutagenesis of mPlum for the purposes of creating a site-specific O6-MeG lesion for the purposes of measuring MGMT activity via Host Cell Reactivation (HCR).DepositorInsertmPlum_C207G/T208C
UseTagsExpressionMammalianMutationC207G + T208CPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-Archon1-KGC-GFP-ER2 (AAV8)
Viral Prep#115892-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-Syn-Archon1-KGC-GFP-ER2 (#115892). In addition to the viral particles, you will also receive purified pAAV-Syn-Archon1-KGC-GFP-ER2 plasmid DNA. Syn-driven Archon1, an opsin-based fluorescent voltage indicator, fused to EGFP. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsGFPAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] (AAV8)
Viral Prep#115893-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] (#115893). In addition to the viral particles, you will also receive purified pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] plasmid DNA. Syn-driven, Cre dependent Archon1, an opsin-based fluorescent voltage indicator, fused to EGFP. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsGFP (Cre-dependent)Available sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2 (AAV5)
Viral Prep#108422-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2 (#108422). In addition to the viral particles, you will also receive purified pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2 plasmid DNA. CAG-driven, Cre dependent Archon1, an opsin-based fluorescent voltage indicator, fused to EGFP. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsEGFP (Cre-dependent)Available sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-GG-hUBC-EBFP2
Plasmid#212314PurposeLentiviral backbone with EBFP2 reporterDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-GG-hUbC-EBPF2-gRNA-TGFB2
Plasmid#185556PurposeLentiviral backbone with EBFP2 reporter and gRNA targeting TGFB2DepositorInsertTGFB2 gRNAs
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-GG-hUbC-EBPF2-gRNA-MFAP2
Plasmid#185555PurposeLentiviral backbone with EBFP2 reporter and gRNA targeting MFAP2DepositorInsertMFAP2 gRNAs
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-GG-hUbC-EBPF2-gRNA-SOX4
Plasmid#185552PurposeLentiviral backbone with EBFP2 reporter and gRNA targeting SOX4DepositorInsertSOX4 gRNAs
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-GG-hUbC-EBPF2-gRNA-FAM13A
Plasmid#185550PurposeLentiviral backbone with EBFP2 reporter and gRNA targeting FAM13ADepositorInsertFAM13A gRNAs
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-GG-hUbC-EBPF2-gRNA-RBMS3
Plasmid#185557PurposeLentiviral backbone with EBFP2 reporter and gRNA targeting RBMS3DepositorInsertRBMS3 gRNAs
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-GG-hUbC-EBPF2-gRNA-DSP
Plasmid#185549PurposeLentiviral backbone with EBFP2 reporter and gRNA targeting DSPDepositorInsertDSP gRNAs
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-GG-hUbC-EBPF2-gRNA-HHIP
Plasmid#185551PurposeLentiviral backbone with EBFP2 reporter and gRNA targeting HHIPDepositorInsertHHIP gRNAs
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-GG-hUbC-EBPF2-gRNA-ADGRG6
Plasmid#185554PurposeLentiviral backbone with EBFP2 reporter and gRNA targeting ADGRG6DepositorInsertADGRG6 gRNAs
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only