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Plasmid#103980PurposePlasmid encoding mGFPmut2DepositorInsertmGFPmut2
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (S65A, V68L, …PromoterproCAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEB1-2ndVal mGFPmut2
Plasmid#104033PurposePlasmid encoding 2ndVal mGFPmut2DepositorInsert2ndVal mGFPmut2
UseLow copyTagsExpressionBacterialMutationmGFPmut2 with valine inserted in 2nd positionPromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I T7
Plasmid#208191Purposeframeshift mistranslation, T7DepositorInsertsGFPmut2
mScarlet-I
UseReporterTagsExpressionBacterialMutationFrameshift insertion 6TTTTTTTPromoterPtet+dnaK P1Available sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I A5
Plasmid#208192Purposeframeshift mistranslation, A5DepositorInsertsGFPmut2
mScarlet-I
UseReporterTagsExpressionBacterialMutationFrameshift insertion 6AAAAAPromoterPtet+dnaK P1Available sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I A4
Plasmid#208193Purposeframeshift mistranslation, A4DepositorInsertsGFPmut2
mScarlet-I
UseReporterTagsExpressionBacterialMutationFrameshift insertion 6AAAAPromoterPtet+dnaK P1Available sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I
Plasmid#208183Purposemistranslation GFP and mScarlet-I positive controlDepositorInsertsGFPmut2
mScarlet-I
UseReporterTagsExpressionBacterialMutationPromoterPtet+dnaK P1Available sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2(ΔmScarlet)
Plasmid#208185PurposeGFP positive, mScarlet-I negative controlDepositorInsertGFPmut2
UseReporterTagsExpressionBacterialMutationPromoterPtet+dnaK P1Available sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I TAG
Plasmid#208188Purposenonsense mistranslation, TAG stopDepositorInsertsGFPmut2
mScarlet-I
UseReporterTagsExpressionBacterialMutationInsertion 6TAG*(stop) 7AGT(Ser)PromoterPtet+dnaK P1Available sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I TGA
Plasmid#208189Purposenonsense mistranslation, TGA stopDepositorInsertsGFPmut2
mScarlet-I
UseReporterTagsExpressionBacterialMutationInsertion 6TGA*(stop) 7AGT(Ser)PromoterPtet+dnaK P1Available sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I TGATC
Plasmid#208190Purposenonsense mistranslation, TGA stop variant 2DepositorInsertsGFPmut2
mScarlet-I
UseReporterTagsExpressionBacterialMutationInsertion 6TGA*(stop) 7TCT(Ser)PromoterPtet+dnaK P1Available sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I A6T
Plasmid#208194Purposeframeshift mistranslation, A6TDepositorInsertsGFPmut2
mScarlet-I
UseReporterTagsExpressionBacterialMutationFrameshift insertion 6AAAAAATPromoterPtet+dnaK P1Available sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I C3T
Plasmid#208195Purposeframeshift mistranslation, C3TDepositorInsertsGFPmut2
mScarlet-I
UseReporterTagsExpressionBacterialMutationFrameshift insertion 6CCCTPromoterPtet+dnaK P1Available sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I 6Trp7Ser
Plasmid#208186Purposemistranslation GFP and mScarlet-I positive control with Trp insertionDepositorInsertsGFPmut2
mScarlet-I
UseReporterTagsExpressionBacterialMutationInsertion 6Trp7SerPromoterPtet+dnaK P1Available sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I TAA
Plasmid#208187Purposenonsense mistranslation, TAA stopDepositorInsertsGFPmut2
mScarlet-I
UseReporterTagsExpressionBacterialMutationInsertion 6TAA*(stop) 7AGT(Ser)PromoterPtet+dnaK P1Available sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pZH516
Plasmid#102669PurposeConstitutive expression of GFPmut2; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZH514
Plasmid#102667PurposeConstitutive expression of GFPmut2; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZH521
Plasmid#102674PurposeTetracycline inducible expression of GFPmut2 with constitutive TetR; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZH520
Plasmid#102673PurposeTetracycline inducible expression of GFPmut2 with constitutive TetR; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZH517
Plasmid#102670PurposeTetracycline inducible expression of GFPmut2 using autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZH519
Plasmid#102672PurposeTetracycline inducible expression of GFPmut2 using autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only