Pooled Library Handling and Storage
Addgene recommends the following steps to ensure proper handling and storage of your pooled library for future use. If there is a problem, please contact Addgene within 30 days of receiving your pooled library. For additional information, please see our Help Center.
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Pooled Library Storage
Most libraries from Addgene arrive as a pooled DNA library on blue ice. They may not be frozen. In some cases, such as shipping to China, pooled libraries will arrive at room temperature. The volume and concentration of DNA you receive will depend upon which library you ordered. See your library's Addgene web page for details.
Addgene recommends storage at 4 °C if using the library in less than four months. If you plan to store the library for more than four months before using it, we recommend storage at -20 °C; please minimize the number of freeze/thaw cycles by aliquoting the sample before storage.
A few libraries are shipped on dry ice. These should arrive frozen and should be immediately stored at -80 °C.
Pooled Library Recovery
To access the library we recommend you spin down the tube before opening the lid. You will need to amplify the pooled library you received from Addgene before using it in a screen. Please find an amplification protocol for the specific library you ordered on its Addgene product page.
Pooled Library Datasheet
Any and all information Addgene has about your library can be found on Addgene’s website. Search using the library name or ID number listed on your Order Summary. Alternatively, you can use the links from your Order Confirmation email.
Plasmid Verification
Addgene cannot guarantee that the depositing laboratories have submitted the correct material. Please notify Addgene of any problems within 30 days.
Please visit www.addgene.org and search the catalog number of your pooled library for any recomended protocols from the depositor.
Citations in Future Publications
The pooled library you have received was created by your colleagues. Please acknowledge the Principal Investigator, cite the article listed on the pooled library datasheet, and include the Addgene catalog ID in future publications.
Contact Addgene
Thank you for requesting a pooled library from Addgene. If you have questions or would like to speak with an Addgene scientist, contact us at [email protected].