Mouse Improved Genome-wide Knockout CRISPR Library v2
(Pooled Library #67988)
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Purpose
A gRNA library against 18,424 mouse genes, designed for use in lentivirus.
gRNAs were redesigned using a new design pipeline as well as an improved scaffold.
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Vector Backbone
pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W (Plasmid #67974) - does not express Cas9
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Depositing Labs
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |||
|---|---|---|---|---|---|---|---|
| Pooled Library | 67988 | Mouse Improved Genome-wide KO CRISPR v2† | 1 | Pending | |||
† A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with pKLV2-EF1a-Cas9Bsd-W (Addgene #68343) or with cell lines already expressing Cas9.
Library Details
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SpeciesMouse
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Genes targeted18,424
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gRNAs90,230
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Lentiviral Generation3rd
Library Shipping
Each library is delivered in microcentrifuge tubes on blue ice. A tube's contents will not necessarily be frozen. For best results, minimize freeze/thaw cycles.
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Volume~50 µL
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Concentration20 ng/µL
Resource Information
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Protocols
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Depositor Data
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Terms and Licenses
Academic/Nonprofit TermsIndustry Terms- Not Available to Industry
Trademarks- Zeocin® is an InvivoGen trademark.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Mouse Improved Genome-wide Knockout CRISPR Library v2 was a gift from Kosuke Yusa (Addgene #67988) -
For your References section:
A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia. Tzelepis K, Koike-Yusa H, De Braekeleer E, Li Y, Metzakopian E, Dovey OM, Mupo A, Grinkevich V, Li M, Mazan M, Gozdecka M, Ohnishi S, Cooper J, Patel M, McKerrell T, Chen B, Domingues AF, Gallipoli P, Teichmann S, Ponstingl H, McDermott U, Saez-Rodriguez J, Huntly BJ, Iorio F, Pina C, Vassiliou GS, Yusa K. Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079. PubMed 27760321 (Link opens in a new window)