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Whitehead Lab PYR1 Double Mutant Library
(Pooled Library #1000000181)

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  • Purpose

    This yeast two-hybrid library is a pair of double mutant libraries at 19 positions of the PYR1 gene. L007 (Library 183177) contains all proximal double mutations separated by fewer than eight amino acids. L008 (Library 183178) contains all distant double mutations separated by eight amino acids or greater.

  • Vector Backbone

    pJS646 (Plasmid #183175)

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 1000000181 PYR1 Double Mutant Library 1 $540 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Species
    A. thaliana (mustard weed)
  • Number of mutants
    42,743

Library Shipping

This library will be delivered as two pooled DNA libraries in microcentrifuge tubes on blue ice (tubes labeled L007 and L008). The tubes' contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼20 µL
  • Concentration
    50 ng/µL

Resource Information

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PYR1 Double Mutant Library was a gift from Timothy Whitehead (Addgene #1000000181)

  • For your References section:

    Rapid biosensor development using plant hormone receptors as reprogrammable scaffolds. Beltrán J, Steiner PJ, Bedewitz M, Wei S, Peterson FC, Li Z, Hughes BE, Hartley Z, Robertson NR, Medina-Cucurella AV, Baumer ZT, Leonard AC, Park S-Y, Volkman BF, Nusinow DA, Zhong W, Wheeldon I, Cutler SR, Whitehead TA. Nat Biotechnol. 2022 Jun 20. doi: 10.1038/s41587-022-01364-5. PMID 35726092.
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