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Dual sgRNA CRISPRi Libraries
(Pooled Library #187246, #187247, #187248, #1000000192)

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  • Purpose

    Ultra-compact, human genome-wide CRISPRi library targeting each gene with a single element encoding a dual sgRNA cassette.

  • Vector Backbone

    pJR104 (Plasmid #187243) - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 1000000192 Three tubes, each with a third of the library (Library 1-2, 3-4, and 5-6) in backbone pJR104†
  • #187246 (Library 1-2)
  • #187247 (Library 3-4)
  • #187248 (Library 5-6)
 1 $1300 Add to Cart
Pooled Library 187246 hCRISPRi_dual_1_2 pooled library† 1 $540 Add to Cart
Pooled Library 187247 hCRISPRi_dual_3_4 pooled library† 1 $540 Add to Cart
Pooled Library 187248 hCRISPRi_dual_5_6 pooled library† 1 $540 Add to Cart
Available to Academic and Nonprofits Only

  • † A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with Zim3-dCas9 (Addgene #188768 or #188777).

Library Details

  • Genes/gRNAs/plasmids
    22,580 dual sgRNA elements
  • Controls
    1,026 dual sgRNA control elements
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼20 µL
  • Concentration
    50 ng/µL

Resource Information

Depositor Comments

User instructions and analysis scripts are available in the lab's GitHub repository (Link opens in a new window).

Please visit https://doi.org/10.1101/2022.07.13.499814 (Link opens in a new window) for bioRxiv preprint.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Dual sgRNA CRISPRi Libraries were a gift from Jonathan Weissman (Addgene #1000000192)
    Dual sgRNA CRISPRi Library 1-2 was a gift from Jonathan Weissman (Addgene #187246)
    Dual sgRNA CRISPRi Library 3-4 was a gift from Jonathan Weissman (Addgene #187247)
    Dual sgRNA CRISPRi Library 5-6 was a gift from Jonathan Weissman (Addgene #187248)

  • For your References section:

    Maximizing CRISPRi efficacy and accessibility with dual-sgRNA libraries and optimal effectors. Replogle JM, Bonnar JL, Pogson AN, Liem CR, Maier NK, Ding Y, Russell BJ, Wang X, Leng K, Guna A, Norman TM, Pak RA, Ramos DM, Ward ME, Gilbert LA, Kampmann M, Weissman JS, Jost M. eLife. 2022 Dec 28;11:e81856. doi: 10.7554/eLife.81856. PubMed 36576240 (Link opens in a new window)
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