Wei Human lncRNA Splicing-targeting CRISPR Library
(Pooled Library #119977)

• Purpose:

  This CRISPR library is designed to target the 5' or 3' splice sites of lncRNAs in genome-scale to knock out the targeted lncRNAs.

• Vector Backbone:

  pLenti-sgRNA(lib)-puro (Plasmid #119976) - does not express Cas9

• Depositing Labs: Wensheng Wei
• Publication: Liu et al. Nat Biotechnol. 2018 Nov 5. pii: nbt.4283. doi: 10.1038/nbt.4283.

Library Details

• Species: Human
• lncRNAs targeted: 10,996
• gRNAs: 126,773
• Controls: 500 non-targeting, 350 ribosomal gene-targeting
• Lentiviral Generation: 3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

• Volume: ∼20 µL
• Concentration: 50 ng/µL

Resource Information

• Protocols:
  • Library Protocols (DOCX, 89 KB)
  • Source Codes for lncRNA Screen Analysis (ZIP, 2.3 MB)
• Depositor Data:
  • List of gRNAs (XLSX, 6.6 MB)
  • NGS Read Counts (XLSM, 5.2 MB)

Terms and Licenses

Academic/Nonprofit Terms:

• UBMTA

Industry Terms:

• Not Available to Industry

Trademarks:

• Zeocin® is an InvivoGen trademark.

Depositor Comments

The read distribution of the two biologically independent samples of the control library in K562 cells showed a high level of correlation (Spearman Corr. = 0.94). Sequencing data from the CRISPR screen can be accessed in NCBI Short Read Archive (SRA) with the accession number SRP157958 under BioProject ID PRJNA486076.