Wei Human lncRNA Splicing-targeting CRISPR Library
(Pooled Library #119977)
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Purpose
This CRISPR library is designed to target the 5' or 3' splice sites of lncRNAs in genome-scale to knock out the targeted lncRNAs.
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Vector Backbone
pLenti-sgRNA(lib)-puro (Plasmid #119976) - does not express Cas9
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Depositing Labs
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | ||
|---|---|---|---|---|---|---|
| Pooled Library | 119977 | gRNA pooled library† | 1 | $430 | Add to Cart | |
† A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with cell lines expressing Cas9.
Library Details
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SpeciesHuman
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lncRNAs targeted10,996
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gRNAs126,773
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Controls500 non-targeting, 350 ribosomal gene-targeting
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Lentiviral Generation3rd
Library Shipping
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.
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Volume∼20 µL
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Concentration50 ng/µL
Resource Information
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Protocols
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Depositor Data
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Terms and Licenses
Academic/Nonprofit TermsIndustry Terms- Not Available to Industry
Trademarks- Zeocin® is an InvivoGen trademark.
Depositor Comments
The read distribution of the two biologically independent samples of the control library in K562 cells showed a high level of correlation (Spearman Corr. = 0.94). Sequencing data from the CRISPR screen can be accessed in NCBI Short Read Archive (SRA) with the accession number SRP157958 under BioProject ID PRJNA486076.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Human lncRNA Splicing-targeting CRISPR library was a gift from Wensheng Wei (Addgene #119977) -
For your References section:
Genome-wide screening for functional long noncoding RNAs in human cells by Cas9 targeting of splice sites. Liu Y, Cao Z, Wang Y, Guo Y, Xu P, Yuan P, Liu Z, He Y, Wei W. Nat Biotechnol. 2018 Nov 5. pii: nbt.4283. doi: 10.1038/nbt.4283. PubMed 30395134 (Link opens in a new window)