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Turner Lab Mouse messenger-RBP sgRNA Library
(Pooled Library #169082)

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Item Catalog # Description Quantity Price (USD)
Pooled Library 169082 Mouse messenger-RBP sgRNA Library† 1 $430 Add to Cart
Available to Academic and Nonprofits Only

  • † A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with lentiCas9-Blast (Addgene #52962) or established Cas9 mouse models.

Library Details

  • Species
    Mouse
  • Genes targeted
    1,300
  • gRNAs
    13,500
  • Controls
    500 non-targeting sgRNAs

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼20 µL
  • Concentration
    50 ng/µL

Resource Information

Depositor Comments


Figure is composed of two panels. Panel A shows maps of the vector backbone and a sgRNA library vector. The vector backbone map contains the hU6 promoter, ccdb toxin, iScaffold, PGK promoter, and Puro-T2A-CD90.1. The sgRNA library vector contains the same elements but the ccdb toxin box has been replaced by sgRNA seed. Panel B shows a bar plot where the x axis represents the log transformed number of mapped reads per sgRNA and spans from 0 to 20, and the y axis represents sgRNA counts and spans from 0 to 3000. Bars show a normal distribution from 7 to 11 number of mapped reads in the x axis, peaking at a number between 2500 and 3000 sgRNA counts in the y axis. Figure text reads 98.8% in 16-fold range.

Figure 1: Mouse messenger-RBP Library Overview. A) Schematic representation of vector backbone and retrovirus sgRNA library components. The vector backbone encodes ccdB toxin gene for cloning (replaced by sgRNA seed sequence), an improved sgRNA scaffold that lacks four consecutive thymine bases and has an extended duplex region, and puromycin resistance and CD90.1 (Thy1.1) selection markers. B) Distribution of the representation of sgRNAs in the library is normally distributed and tightly distributed.

Please visit https://doi.org/10.1101/2021.07.21.453193 (Link opens in a new window) for bioRxiv preprint.

See also Dang, Y. et al. Optimizing sgRNA structure to improve CRISPR-Cas9 knockout efficiency. Genome Biol. 2015 Dec 15:16:280. doi: 10.1186/s13059-015-0846-3 (Link opens in a new window).

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mouse messenger-RBP sgRNA Library was a gift from Martin Turner (Addgene #169082)

  • For your References section:

    A functional screen of RNA binding proteins identifies genes that promote or limit the accumulation of CD138+ plasma cells. Turner DJ, Saveliev A, Salerno F, Matheson LS, Screen M, Lawson H, Wotherspoon D, Kranc KR, Turner M. eLife 2022;11:e72313. doi: 10.7554/eLife.72313 PubMed 35451955 (Link opens in a new window)
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