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Yeast Inducible CRISPRi Library – Ampl133
(Pooled Library #161829)

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  • Purpose

    This inducible genome-wide yeast CRISPRi library targets S. cerevisiae genes with 6-12 guide RNAs per gene. gRNA expression is induced using anhydrous tetracycline (ATc).

  • Vector Backbone

    ampl43 (Plasmid #161830)

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 161829 Yeast Inducible CRISPRi Library – Ampl133 1 $430 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Species
    S. cerevisiae
  • Genes targeted
    6,685
  • gRNAs
    51,681
  • Controls
    500 randomly shuffled synthetic gRNAs
  • Selectable Marker
    HIS3

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼20 µL
  • Concentration
    50 ng/µL

Resource Information

Depositor Comments

The Tavazoie Lab provides scripts to process CRISPRi screen data: Tavalab Github Repository

The depositing lab's sequencing protocol can be found in the methods and materials section of the associated publication.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Yeast Inducible CRISPRi Library - Ampl133 was a gift from Saeed Tavazoie (Addgene #161830)

  • For your References section:

    An inducible CRISPR interference library for genetic interrogation of Saccharomyces cerevisiae biology. Momen-Roknabadi A, Oikonomou P, Zegans M, Tavazoie S. Commun Biol. 2020 Nov 27;3(1):723. doi: 10.1038/s42003-020-01452-9. PubMed 33247197
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