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Human Cas13 lncRNA Library
(Pooled Library #227014)

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  • Purpose

    The human CRISPR-Cas13 lentiviral pooled library targets long noncoding RNAs (lncRNAs) that are highly expressed in human tissues for transcriptome-wide knockdown screens. It also targets the protein-coding genes nearest to these lncRNAs to determine cis-regulatory functions.

  • Vector Backbone

    pLentiRNAGuide_001 - hU6-RfxCas13d-DR1-BsmBI-EFS-Puro-WPRE (Plasmid #138150) - does not express Cas13

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 227014 Human Cas13 lncRNA Library† 1 $540 Add to Cart
Available to Academic and Nonprofits Only

  • † Must be used in cells that express RfxCas13d (not Cas9); a Cas13 plasmid is NOT included with this item and will have to be ordered separately. The depositing lab recommends using pLentiRNACRISPR_007 - TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast (Addgene #138149) to first make a RfxCas13d-expressing cell line.

Library Details

  • Species
    Human
  • Genes targeted
    6,199 lncRNAs and 4,390 protein-coding genes
  • gRNAs
    75,184 total gRNAs (8 per lncRNA, 6 per protein-coding gene)
  • Controls
    1,000 non-targeting gRNAs (negative controls), 1,000 gRNAs targeting essential protein-coding genes (positive controls)
  • Lentiviral generation
    3rd

Library Shipping

Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼20 µL
  • Concentration
    50 ng/µL

Resource Information

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human Cas13 lncRNA Library was a gift from Neville Sanjana (Addgene #227014)

  • For your References section:

    Transcriptome-scale RNA-targeting CRISPR screens reveal essential lncRNAs in human cells. Liang WW, Müller S, Hart SK, Wessels HH, Méndez-Mancilla A, Sookdeo A, Choi O, Caragine CM, Corman A, Lu L, Kolumba O, Williams B, Sanjana NE. Cell. 2024 Dec 26;187(26):7637-7654.e29. doi: 10.1016/j.cell.2024.10.021. PubMed 39532094 (Link opens in a new window)
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