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Human CRISPR Knockout Pooled Libraries (Enriched Sub-pools)
(Pooled Libraries #51043, #51044, #51045, #51046, #51047, #51048)

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  • Purpose

    Human CRISPR knockout pooled libraries that have been separated into six distinct, enriched sub-pools.

    Note that while each sub-pool is enriched for their respective target sgRNAs, they are not exclusive and elements of the entire library may be present in each sub-pool.

  • Vector Backbone

    pLX-sgRNA (Plasmid #50662) - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 51043 Unknown function gRNA sub-pool library † 1 $430 Add to Cart
Pooled Library 51044 Kinase gRNA sub-pool library † 1 $430 Add to Cart
Pooled Library 51045 Ribosomal protein gRNA sub-pool library † 1 $430 Add to Cart
Pooled Library 51046 Cell cycle proteins gRNA sub-pool library † 1 $430 Add to Cart
Pooled Library 51047 Nuclear proteins gRNA sub-pool library † 1 $430 Add to Cart
Pooled Library 51048 Control targets (most diverse) gRNA sub-pool library † 1 $380 Add to Cart
Available to academic and nonprofits only.

  • † A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with pCW-Cas9 (Addgene#50661) or otherwise with cell lines already expressing Cas9.

Library Details

  • Species
    Human
  • Genes targeted
    Varies
  • gRNAs
    Varies
  • Controls
    See above Pooled Library #51048 enriched control sub-pool
  • Lentiviral Generation
    3rd

Library Shipping

Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼20 µL
  • Concentration
    50 ng/µL

Resource Information

Depositor Comments

IMPORTANT NOTE: Though each sub-pool is enriched for its respective target sgRNAs, they are not exclusive and elements of the entire library may be present in each sub-pool.

There are two lentiviral vectors associated with this paper, one expressing Cas9 ( pCW-Cas9 ) and the sgRNA backbone ( pLX-sgRNA ).

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human CRISPR enriched pooled library was a gift from David Sabatini and Eric Lander (Addgene #51043)
    Human CRISPR enriched pooled library was a gift from David Sabatini and Eric Lander (Addgene #51044)
    Human CRISPR enriched pooled library was a gift from David Sabatini and Eric Lander (Addgene #51045)
    Human CRISPR enriched pooled library was a gift from David Sabatini and Eric Lander (Addgene #51046)
    Human CRISPR enriched pooled library was a gift from David Sabatini and Eric Lander (Addgene #51047)
    Human CRISPR enriched pooled library was a gift from David Sabatini and Eric Lander (Addgene #51048)

  • For your References section:

    Genetic screens in human cells using the CRISPR-Cas9 system . Wang T, Wei JJ, Sabatini DM, Lander ES. Science. 2014 Jan 3;343(6166):80-4. doi: 10.1126/science.1246981. Epub 2013 Dec 12. 10.1126/science.1246981 PubMed 24336569
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