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Human CRISPR Metabolic Gene Knockout Library
(Pooled Library #110066)

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  • Purpose

    This lentiviral CRISPR library targets 2,981 human metabolic genes with 29,790 guide RNAs. The library contains ~10 gRNAs per gene.

  • Vector Backbone

    lentiCRISPR v1 (Plasmid #49535) - expresses Cas9

    Note: This plasmid has been discontinued, as an updated version is available. For confirmation of screen hits, Addgene encourages recipient scientists to use the updated lentiCRISPR v2 (Plasmid #52961), which can be requested separately.

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 110066 gRNA pooled library 1 $430 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Species
    Human
  • Genes targeted
    2,981
  • gRNAs
    30,290
  • Controls
    500 intergenic
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼20 µL
  • Concentration
    50 ng/µL

Resource Information

Depositor Comments

Library screening is performed by comparing sgRNA abundance of an initial cell population and a final cell population after cell growth, using massively parallel sequencing.

Figure 1: Overview of library screening process.

When performing sgRNA validation in a Cas9-expressing cell line, the depositing laboratory recommends using plasmid pLenti-sgRNA (Addgene #71049).

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human CRISPR Metabolic Gene Knockout library was a gift from David Sabatini (Addgene #110066)

  • For your References section:

    An Essential Role of the Mitochondrial Electron Transport Chain in Cell Proliferation Is to Enable Aspartate Synthesis. Birsoy K, Wang T, Chen WW, Freinkman E, Abu-Remaileh M, Sabatini DM. Cell. 2015 Jul 30;162(3):540-51. doi: 10.1016/j.cell.2015.07.016. PubMed 26232224 (Link opens in a new window)
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