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LARRYv2 Libraries
(Pooled Library #233213, #233214, #233215, #233216, #233697)

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  • Purpose

    These plasmid libraries are used to produce lentiviral barcoding particles to barcode mammalian cells for clonal and state-fate analysis using single-cell RNA sequencing. The lineage and RNA recovery (LARRY) version 2 (v2) architecture follows an EF1a-Reporter-WPRE-barcode-pA structure, allowing the 34-bp barcode to be expressed and detected in the poly-adenylated mRNA.

  • Vector Backbone

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 233213 LARRYv2-T-Sapphire library 1 1 $325 Add to Cart
Pooled Library 233214 LARRYv2-T-Sapphire library 2 1 $325 Add to Cart
Pooled Library 233215 LARRYv2-EGFP library 1 1 $325 Add to Cart
Pooled Library 233216 LARRYv2-EGFP library 2 1 $325 Add to Cart
Pooled Library 233697 LARRYv2-mScarlet library 1 $325 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Number of Barcodes
    >50 million
  • Lentiviral generation
    3rd

Library Shipping

Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼20 µL
  • Concentration
    60 ng/µL

Resource Information

Depositor Comments

Schematic showing the construction of pLARRYv2 pooled libraries. Each backbone plasmid includes 5' LTR, EF1alpha promoter, fluorescent marker (T-Sapphire, EGFP, or mScarlet), WPRE, EcoRV site, and 3' LTR with poly-A signal (pA). The Barcode oligos used for cloning each library are indicated: GTTCCA-barcode for LARRYv2 T-Sapphire library 1, TGCTAA-barcode for LARRYv2 T-Sapphire library 2, TCCAGT-barcode for LARRYv2 EGFP library 1, TGATTG-barcode for LARRYv2 EGFP library 2, and AAGATT-barcode for LARRYv2 mScarlet library.

Figure 1: LARRYv2 Library components. The pairs of libraries with T-Sapphire and EGFP incorporate different barcode oligos as indicated.

Please visit https://doi.org/10.1101/2024.05.14.594084 (Link opens in a new window) for bioRxiv preprint.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LARRYv2-T-Sapphire library 1 was a gift from Alejo Rodriguez-Fraticelli (Addgene #233213)
    LARRYv2-T-Sapphire library 2 was a gift from Alejo Rodriguez-Fraticelli (Addgene #233214)
    LARRYv2-EGFP library 1 was a gift from Alejo Rodriguez-Fraticelli (Addgene #233215)
    LARRYv2-EGFP library 2 was a gift from Alejo Rodriguez-Fraticelli (Addgene #233216)
    LARRYv2-mScarlet library was a gift from Alejo Rodriguez-Fraticelli (Addgene #233697)

  • For your References section:

    Pre-existing stem cell heterogeneity dictates clonal responses to the acquisition of leukemic driver mutations. Singh I, Fernandez Perez D, Sánchez PS, Rodriguez-Fraticelli AE. Cell Stem Cell. 2025 Feb 19:S1934-5909(25)00012-8. doi: 10.1016/j.stem.2025.01.012. PMID 40010350 (Link opens in a new window)
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