Rock Lab Mycobacterium tuberculosis CRISPRi Library
(Pooled Library #163954)

• Purpose:

  This CRISPR repression library inhibits expression of open reading frames (ORFs) and non-coding RNAs in M. tuberculosis (RLC12).

  The anhydrotetracycline (ATc)-inducible CRISPRi library was constructed in an L5-integrating backbone. It can be used in competitive growth experiments to reveal gene vulnerability.

• Vector Backbone:

  pIRL2 (Plasmid #163631)

• Depositing Labs: Jeremy Rock
• Publication: Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00824-2. doi: 10.1016/j.cell.2021.06.033.

Library Details

• Species: M. tuberculosis
• Genes targeted: 4,052
• gRNAs: 96,700
• Controls: 1,658

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

• Volume: ∼20 µL
• Concentration: 50 ng/µL

Resource Information

• Protocols:
  • Library Transformation and Sequencing Protocol (DOCX, 16 KB)
• Depositor Data:
  • RLC12 Targets and Guide Sequences (CSV, 10 MB)
• Articles Citing this Pooled Library:
  • RLC12 gRNA Library (#163954)

Terms and Licenses

Academic/Nonprofit Terms:

• UBMTA

Industry Terms:

• Not Available to Industry

Trademarks:

• Zeocin® is an InvivoGen trademark.

Depositor Comments

Code and methods for vulnerability studies with these libraries can be found at the Rock Lab's GitHub repository.

See the STAR methods in PubMed 34297925 for PCR and sequencing protocols.