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  • Mouse Cardiac Transcriptional Regulators CRISPR Knockout Library

Pu Lab - Mouse Cardiac Transcriptional Regulators CRISPR Knockout Library
(Pooled Library #138015)

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  • Purpose

    This AAV CRISPR knockout library is designed to investigate cardiac gene regulation.

    Guide RNAs target transcriptional regulators expressed in mouse cardiomyocytes and a subset of GATA4-regulated genes.

  • Vector Backbone

    AAV-U6-gRNA-TnT-Cre (Plasmid #132551) - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 138015 gRNA pooled library† 1 $380 Add to Cart
Available to Academic and Nonprofits Only

  • † A Cas9 plasmid is NOT included with this item. The AAV backbone expresses a Cre-recombinase and can be used with Cre-activatable Cas9.

Library Details

  • Species
    Mouse
  • Genes targeted
    2,444
  • gRNAs
    14,671
  • Controls
    7 gRNAs target human genome

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼20 µL
  • Concentration
    25 ng/µL

Resource Information

Depositor Comments

Workflow for selecting genes for the library: 1,894 transcriptional regulators, 259 genes differentially regulated in GATA4/6 KO, 291 genes with straong adjacent binding of GATA4, and 7 negative control gRNAs (human targeting). In total, 2,444 genes are targeted with 14,671 gRNAs (6 gRNAs/gene).

Figure 1: Components of the Mouse Cardiac Transcriptional Regulators CRISPR Knockout Library.

Please visit https://doi.org/10.1101/808402 (Link opens in a new window) for bioRxiv preprint.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mouse Cardiac Transcriptional Regulators CRISPR Knockout Library was a gift from William Pu (Addgene #138015)

  • For your References section:

    In vivo CRISPR screening identifies RNF20/40 as epigenetic regulators of cardiomyocyte maturation. VanDusen NJ, Lee JY, Gu W, Sethi I, Zheng Y, King JS, Zhou P, Suo S, Guo Y, Ma Q, Yuan G, Pu WT. bioRxiv 2019, 808402v1. doi: https://doi.org/10.1101/808402 (Link opens in a new window).
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