Human Lipid Droplet and Metabolism Library
(Pooled Library #191535)
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Purpose
Lentiviral CRISPR library targeting 1,196 genes related to lipid droplet biology and cell metabolism. The library contains 13,920 elements (~10 guide RNAs per gene) including 2,000 negative control gRNAs. This library will be useful to researchers in the fields of lipid biology and metabolism.
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Vector Backbone
pMCB320 (Plasmid #89359) - does not express Cas9
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Depositing Labs
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | ||
|---|---|---|---|---|---|---|
| Pooled Library | 191535 | Human Lipid Droplet and Metabolism Library† | 1 | $430 | Add to Cart | |
† A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used with cells expressing Cas9 or a Cas9-expressing plasmid such as lentiCas9-Blast (Addgene #52962).
Library Details
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SpeciesHuman
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Genes targeted1,196
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gRNAs13,920
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Controls1,000 non-targeting and 1,000 safe guides targeting genomic locations without annotated functions
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Lentiviral Generation3rd
Library Shipping
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.
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Volume∼20 µL
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Concentration50 ng/µL
Resource Information
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Protocols
- Amplification Protocol (PDF, 280 KB)
- For library sequencing, please see Deans et al., (2016) (Link opens in a new window)
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Depositor Data
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Articles Citing this Pooled Library
Terms and Licenses
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Academic/Nonprofit TermsIndustry Terms
- Not Available to Industry
Trademarks- Zeocin® is an InvivoGen trademark.
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Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.08.27.505556v1 (Link opens in a new window)for bioRxiv preprint.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Human Lipid Droplet and Metabolism library was a gift from James Olzmann (Addgene #191535) -
For your References section:
Parallel CRISPR-Cas9 screens identify mechanisms of PLIN2 and lipid droplet regulation. Roberts MA, Deol KK, Mathiowetz AJ, Lange M, Leto DE, Stevenson J, Hashemi SH, Morgens DW, Easter E, Heydari K, Nalls MA, Bassik MC, Kampmann M, Kopito RR, Faghri F, Olzmann JA. Dev Cell. 2023 Jul 18:S1534-5807(23)00331-3. doi: 10.1016/j.devcel.2023.07.001. PubMed 37494933