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Advanced Catalogue of Epigenetic Regulators (ACER)
(Pooled Library #226117)

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  • Purpose

    The Advanced Catalogue of Epigenetic Regulators (ACER) library consists 748 traditional and newly predicted epigenetic regulators using epigenetic network analyses that integrated publicly available gene codependency (DepMap) and protein-protein interaction data (BioPlex). It allows scientists to study how different epigenetic proteins influence gene activity and contribute to diseases. Scientists can utilize this library to discover the contributions of specific epigenetic regulators in gene expression, chromatin dynamics, and disease mechanisms, facilitating the identification of novel therapeutic targets.

  • Vector Backbone

    LRG2.1_Neo (Plasmid #125593) - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 226117 ACER pooled library† 1 Pending Back-ordered
Available to Academic and Nonprofits Only

  • † A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with lentiCas9-blast (Addgene #52962) or any other plasmid(s) or cell lines expressing Cas9.

Library Details

  • Species
    Human
  • Genes targeted
    758
  • gRNAs
    8,205
  • Controls
    3 control genes (AAVS1, CCR5, and ROSA26), 625 sgRNAs in total
  • Lentiviral Generation
    3rd

Library Shipping

Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼20 µL
  • Concentration
    25 ng/µL

Resource Information

Depositor Comments

The schematic is shown as a flow chart, starting with 333 classic epigenetic modulators and adds the interactions and co-dependency genes to end with the 758 extended epigenetic regulators.
Figure 1: Schematic of ACER library design. Classical epigenetic modulators (n=333) were extended by including their 343 interactors from the Bioplex Interactome and 102 co-dependency genes from DepMap dataset, resulting in a collection of 758 epigenetic regulator genes (from Zhou et al., 2024).
How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human CRISPR Metabolic Gene Knockout library was a gift from Rui Lu (Addgene #226117)

  • For your References section:

    Epigenetic Regulation of Non-canonical Menin Targets Modulates Menin Inhibitor Response in Acute Myeloid Leukemia. Zhou X, Zhang L, Aryal S, Veasey V, Tajik A, Restelli C, Moreira S, Zhang P, Zhang Y, Hope K, Zhou Y, Cheng C, Bhatia R, Lu R. 2024 Aug 16:blood.2023023644. doi: 10.1182/blood.2023023644. PubMed 39158067
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