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Human Transcription Factor Knockout Library
(Pooled Library #162275)

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  • Purpose

    This lentiviral CRISPR knockout library targets 1,639 human transcription factors for comprehensive loss-of-function screens.

  • Vector Backbone

    lentiGuide-Puro (Plasmid #52963) - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 162275 Lenti-human-TF-gRNA-puromycin† 1 $380 Add to Cart
Available to Academic and Nonprofits Only

  • † A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Plasmid(s) such as lentiCas9-Blast (Addgene #52962) or cell lines expressing SpCas9 can be used.

Library Details

  • Species
    Human
  • Genes targeted
    1,639
  • gRNAs
    11,364
  • Controls
    100 non-targeting gRNAs
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼20 µL
  • Concentration
    50 ng/µL

Resource Information

Depositor Comments

Schematic of the generation and use of the human transcription factor CRISPR knockout library. Library contains 1,639 transcription factors with an average of seven gRNAs per gene and 100 negative controls. Concurrently, a reporter cell line is made using a knock in reporter and Cas9. The library is introduced to the cell line and sorted by FACS based on the reporter. DNA is collected and sequenced using deep sequencing to identify enriched gRNAs.

Figure 1: Overview of the human transcription factor CRISPR knockout library.

The depositing lab recommends using MAGeCK/MAGeCK-VISPR algorithms (Link opens in a new window) and DESeq2 (Link opens in a new window) for analysis.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lenti-human-TF-gRNA-puromycin library was a gift from Chunliang Li (Addgene #162275)

  • For your References section:

    Functional interrogation of HOXA9 regulome in MLLr leukemia via reporter-based CRISPR/Cas9 screen. Zhang H, Zhang Y, Zhou X, Wright S, Hyle J, Zhao L, An J, Zhao X, Shao Y, Xu B, Lee HM, Chen T, Zhou Y, Chen X, Lu R, Li C. eLife. 2020 Oct 1;9. pii: 57858. doi: 10.7554/eLife.57858. PubMed 33001025 (Link opens in a new window)
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