Superti-Furga Human SLC Overexpression Library
(Pooled Library #213694)

• Purpose:

  The library can be used to test the impact of SLC transporters on biological processes. Since it is an overexpression library with around 450 codon-optimized cDNAs, it is suitable for medium-throughput approaches and cell systems where the number of cells cannot easily be scaled up. The library is inducible, allowing for the use of uninduced cell pools as controls, instead of the plasmid library. The barcodes are associated with Unique Molecular Identifiers (UMIs) to allow tracking of individual clones.

• Vector Backbone:

  pCW57.1

• Depositing Labs: Giulio Superti-Furga
• Publication: Human SLC Overexpression Library, Unpublished.

Library Details

• Species: Human
• Genes targeted: 467 barcoded inserts
• Insert size: 327–4,593 bp
• Vector Type: Mammalian Expression, Lentiviral
• Lentiviral Generation: 3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

• Volume: ∼15 µL
• Concentration: 50 ng/µL

Resource Information

• Protocols:
  • Human SLC overexpression amplification protocol (DOCX, 18 KB)
  • Preparation of Human SLC overexpression library protocol (DOCX, 55 KB)
• Depositor Data:
  • pLIX_OE_MAGeCK_library_file (TXT, 14 KB)
  • cDNAs and barcode counts (XLSX, 271 KB)
  • Count distribution (TIF, 108 KB)

Terms and Licenses

Academic/Nonprofit Terms:

• UBMTA

Industry Terms:

• Not Available to Industry

Trademarks:

• Zeocin® is an InvivoGen trademark.

Depositor Comments

The Superti-Furga lab used a simple workflow on Galaxy that is available from the lab upon request. However, the exact parameters will depend on the sequencing mode so it cannot be applied by default.