Superti-Furga Lab Human SLC Activation Library
(Pooled Library #132561)
-
Purpose
This CRISPR transcriptional activation library targets the human solute carrier family of proteins.
-
Vector Backbone
lenti sgRNA(MS2)_zeo backbone (Plasmid #61427) - does not express Cas9
-
Depositing Labs
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | ||
|---|---|---|---|---|---|---|
| Pooled Library | 132561 | Human SLC SAM Library† | 1 | $380 | Add to Cart | |
† A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Use in conjunction with lenti dCAS-VP64_Blast (Addgene #61425) and lenti MS2-P65-HSF1_Hygro (Addgene #61426), pPB-R1R2_EF1aVP64dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA (Addgene #113341) or any other plasmid(s) or cell lines expressing both dCas9-VP64 and MS2-p65-HSF1.
Library Details
-
SpeciesHuman
-
Genes targeted388
-
gRNAs3,342
-
Controls120 non-targeting controls
-
Lentiviral Generation3rd
Library Shipping
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.
-
Volume∼20 µL
-
Concentration50 ng/µL
Resource Information
-
Protocols
-
Depositor Data
-
Terms and Licenses
Academic/Nonprofit TermsIndustry Terms- Not Available to Industry
Trademarks- Zeocin® is an InvivoGen trademark.
Depositor Comments
The library results can be analyzed with several packages, including the MAGeCK algorithms or R/DESeq.
The Research Empowerment on Solute Carriers (ReSOLUTE) plasmid collection contains codon-optimized human SLCs in Gateway-compatible pENTR vectors and may be a useful resource for researchers using this library.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
Human SLC Activation Library was a gift from Giulio Superti-Furga (Addgene #132561) -
For your References section:
Gain-of-function genetic screens in human cells identify SLC transporters overcoming environmental nutrient restrictions. Rebsamen M, Girardi E, Sedlyarov V, Scorzoni S, Papakostas K, Vollert M, Konecka J, Guertl B, Klavins K, Wiedmer T, Superti-Furga G Life Sci Alliance. 2022 Sep 16;5(11):e202201404. doi: 10.26508/lsa.202201404. PubMed 36114003